European Food Research and Technology

, Volume 230, Issue 6, pp 885–891

Quantitative and qualitative analysis of non-Saccharomyces yeasts in spontaneous alcoholic fermentations

  • E. Ocón
  • A. R. Gutiérrez
  • P. Garijo
  • C. Tenorio
  • I. López
  • R. López
  • P. Santamaría
Original Paper

DOI: 10.1007/s00217-010-1233-7

Cite this article as:
Ocón, E., Gutiérrez, A.R., Garijo, P. et al. Eur Food Res Technol (2010) 230: 885. doi:10.1007/s00217-010-1233-7

Abstract

In this study, we looked at the yeast population present in four spontaneous alcoholic fermentations in the Rioja appellation (D.O.Ca. Rioja, Spain). The study was conducted through the identification of the yeasts via the PCR–RFLP technique of the ITS region of rDNA. In a first harvest, the qualitative diversity of the species present in spontaneous alcoholic fermentation was studied, and in a second harvest, their quantitative significance. In spontaneous fermentations, 17 different yeast species were found, although only two of them, Candida stellata and Kloeckera apiculata, as well as Saccharomyces cerevisiae, appeared in major proportions during fermentation. The significance of the non-Saccharomyces yeasts during the spontaneous alcoholic fermentation was conditioned by the presence of Saccharomyces cerevisiae in the medium. Species not cited in literature in connection with winemaking and yeasts associated with wines spoilage have been detected in all the alcoholic fermentations carried out in the qualitative study.

Keywords

YeastsAlcoholic fermentationNon-SaccharomycesPCR–RFLP

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • E. Ocón
    • 1
  • A. R. Gutiérrez
    • 1
  • P. Garijo
    • 2
  • C. Tenorio
    • 2
  • I. López
    • 2
  • R. López
    • 2
  • P. Santamaría
    • 2
  1. 1.ICVV, Instituto de Ciencias de la Vid y el VinoUniversidad de La RiojaLogroñoSpain
  2. 2.ICVV, Instituto de Ciencias de la Vid y el VinoGobierno de La RiojaLogroñoSpain