European Food Research and Technology

, Volume 228, Issue 1, pp 83–91

Polycyclic aromatic hydrocarbons (PAH) in various types of tea

Authors

  • Katja Ziegenhals
    • Max Rubner-Institut (MRI)Federal Research Institute of Nutrition and Food, Analysis Division
    • Max Rubner-Institut (MRI)Federal Research Institute of Nutrition and Food, Analysis Division
  • Karl Speer
    • Institute of Food ChemistryTechnical University of Dresden
Original Paper

DOI: 10.1007/s00217-008-0909-8

Cite this article as:
Ziegenhals, K., Jira, W. & Speer, K. Eur Food Res Technol (2008) 228: 83. doi:10.1007/s00217-008-0909-8

Abstract

For the analysis of the 16 PAH (EFSA-PAH), which are classified as priority for different food groups by the Scientific Committee on Food (SCF) and the Joint FAO/WHO Experts Committee on Food Additives (JECFA) in tea, a sensitive analytical Fast-GC/HRMS method was used. The sample preparation included accelerated solvent extraction (ASE) and the highly automated clean up steps, gel permeation chromatography and solid phase extraction. The analytical parameters, limit of detection (0.01–0.02 μg/kg) and limit of quantification (0.03–0.06 μg/kg), were determined. The repeatability (RSD, n = 3) of different PAH in fruit tea ranged from 0.1 to 11%. It was observed that the total contents of the 16 PAH in tea samples ranged from 14 to 2,662 μg/kg. The analysed tea samples showed an increasing presence of PAH in the following order: herbal and fruit tea (n = 7) < black tea (n = 11) < green tea (n = 11) < white tea (n = 3) < mate-tea (n = 8). The correlation coefficient (R) between BaP and the sum of the 16 EFSA-PAH was established considering the contamination amount in all the 40 tea samples analysed.

Keywords

EFSAFast-GCGC/HRMSPAHTea

Abbreviations

5MC

5-Methylchrysene

ASE

Accelerated solvent extraction

BaA

Benzo[a]anthracene

BaP

Benzo[a]pyrene

BbF

Benzo[b]fluoranthene

BcL

Benzo[c]fluorene

BgP

Benzo[g,h,i]perylene

BjF

Benzo[j]fluoranthene

BkF

Benzo[k]fluoranthene

CPP

Cyclopenta[c,d]pyrene

CHR

Chrysene

D/A

Dichloromethane/acetone

DeP

Dibenzo[a,e]pyrene

DhA

Dibenzo[a,h]anthracene

DhP

Dibenzo[a,h]pyrene

DiP

Dibenzo[a,i]pyrene

DlP

Dibenzo[a,l]pyrene

EFSA

European Food Safety Authority

EPA

Environmental Protection Agency

GPC

Gel permeation chromatography

HRMS

High resolution mass spectrometer

IcP

Indeno[1,2,3-cd]pyrene

IQR

Interquartile range

JECFA

Joint FAO/WHO Experts Committee on Food Additives

KOH

Potassium hydroxide

LLE

Liquid liquid extraction

LOD

Limit of detection

LOQ

Limit of quantification

Na2SO4

Sodium sulphate

PAH

Polycyclic aromatic hydrocarbons

SCF

Scientific Committee on Food

SIM

Selected ion monitoring

SPE

Solid phase extraction

Copyright information

© Springer-Verlag 2008