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Antibacterial and antioxidant properties of Ramulus Cinnamomi using supercritical CO2 extraction

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Abstract

In this study, crude extracts of Ramulus Cinnamomi from supercritical carbon dioxide under various extraction conditions were examined for their antioxidant and antibacterial activity. The extractions were conducted in the range of 4,000–6,000 psi and 40–50 °C, and the solvent to feed ratio of the extraction was 30. The antibacterial activity was tested on the clinical drug-resistant strains, including 27 Acinetobacter baumannii, 20 Pseudomonas aeruginosa and 2 Staphylococcus aureus isolates by the disk diffusion method. The bioassay results indicated that Ramulus Cinnamomi showed obvious antimicrobial activity against the tested strains. This study also found that increasing the temperature and pressure would increase the yield of the supercritical fluid extraction (SFE), even though the best extraction conditions for antibacterial activity were found to be high pressure and low temperature. The minimum inhibitory concentration (MIC) was determined on the crude extract of Ramulus Cinnamomi, indicating that the crude extracts from supercritical extraction showed better antibacterial activity than those obtained by ethanol extraction. Based on the spectrophotometer and bioassay determination, the antimicrobial constituent was identified to be cinnamaldehyde. Time-kill kinetics and scanning electron microscopy (SEM) were employed to monitor the survival characteristics and the changes in morphologies, respectively, of the test microorganisms in the presence of herbal extracts. Moreover, antioxidant activity was evaluated by scavenging of the free radical DPPH. Extracts of Ramulus Cinnamomi provided 50% inhibition at 2 mg/ml concentration. This study will provide valuable information for extraction of the natural bioactive component, cinnamaldehyde, from Ramulus Cinnamomi by supercritical extraction.

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Acknowledgments

Financial support from NSC 94-2515-E-214-003 is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Chemical Engineering, I-Shou University, Kaohsiung, 840, Taiwan, ROC

    Ming-Tsai Liang, Shun-Tai Li & Li-Yeh Chuang

  2. Department of Electronic Engineering, National Kaohsiung University of Applied Sciences, Kaohsiung, 807, Taiwan, ROC

    Cheng-Hong Yang

  3. Department of Plastic Surgery, Chiayi Christian Hospital, Chiayi, 840, Taiwan, ROC

    Cheng-San Yang

  4. Department of Biomedical Science and Environmental Biology and Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan, ROC

    Hsueh-Wei Chang

  5. Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan, ROC

    Hsueh-Wei Chang, Ching-Shen Liu & Thau-Ming Cham

  6. Kaohsiung Municipal Chinese Medical Hospital, Kaohsiung, 80281, Taiwan, ROC

    Ching-Shen Liu

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  1. Ming-Tsai Liang
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Correspondence to Cheng-Hong Yang or Li-Yeh Chuang.

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Liang, MT., Yang, CH., Li, ST. et al. Antibacterial and antioxidant properties of Ramulus Cinnamomi using supercritical CO2 extraction. Eur Food Res Technol 227, 1387–1396 (2008). https://doi.org/10.1007/s00217-008-0857-3

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  • DOI: https://doi.org/10.1007/s00217-008-0857-3

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