European Food Research and Technology

, Volume 227, Issue 5, pp 1287–1297

Alternative DNA amplification methods to PCR and their application in GMO detection: a review

  • Dany Morisset
  • Dejan Stebih
  • Katarina Cankar
  • Jana Zel
  • Kristina Gruden
Review article

DOI: 10.1007/s00217-008-0850-x

Cite this article as:
Morisset, D., Stebih, D., Cankar, K. et al. Eur Food Res Technol (2008) 227: 1287. doi:10.1007/s00217-008-0850-x

Abstract

Nucleic acids, especially DNA, are targets of qualitative and quantitative diagnostics for genetically modified organisms (GMO) in seeds, food- and feedstuff. The amplification of the nucleic acid is an essential step for further analyses of the target sequence. The PCR has been the method of choice for DNA amplification in most laboratories, and its real-time version (qPCR) also enables quantitative analysis of target contents. Despite its numerous advantages, PCR technology has some limitations such as the lack of true multiplexing properties. To alleviate the drawbacks linked to PCR technology, alternative nucleic acid amplification methods with promising characteristics are being developed fast. These methods, their advantages, and the inconveniences, which are not yet resolved are summarized in the paper. Special focus is given to the possibilities of using these alternative methods for GMO detection in future, when expansion of GMOs both in diversity and frequencies will make current GMO detection systems difficult to operate.

Keywords

Alternative methodsDNA amplificationDetectionGenetically modified organismsFood and feed safetyBiotechnology

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Dany Morisset
    • 1
  • Dejan Stebih
    • 1
  • Katarina Cankar
    • 1
  • Jana Zel
    • 1
  • Kristina Gruden
    • 1
  1. 1.Department of Plant Physiology and BiotechnologyNational Institute of BiologyLjubljanaSlovenia