European Food Research and Technology

, Volume 221, Issue 3, pp 452–458

Process stability of Capsicum annuum pectin methylesterase in model systems, pepper puree and intact pepper tissue

Authors

  • Sónia Marília Castro
    • Department of ChemistryUniversidade de Aveiro
  • Ann Van Loey
    • Laboratory of Food TechnologyFaculty of Applied Bioscience and Engineering, Katholieke Universiteit Leuven
  • Jorge Alexandre Saraiva
    • Department of ChemistryUniversidade de Aveiro
  • Chantal Smout
    • Laboratory of Food TechnologyFaculty of Applied Bioscience and Engineering, Katholieke Universiteit Leuven
    • Laboratory of Food TechnologyFaculty of Applied Bioscience and Engineering, Katholieke Universiteit Leuven
Original Paper

DOI: 10.1007/s00217-005-1205-5

Cite this article as:
Castro, S.M., Van Loey, A., Saraiva, J.A. et al. Eur Food Res Technol (2005) 221: 452. doi:10.1007/s00217-005-1205-5

Abstract

Process stability studies towards temperature and/or pressure on pepper pectin methylesterase (PME) were carried out in different systems (purified form, crude extract, pepper pieces and puree) at pH 5.6. Within the temperature range studied (22–80 °C, 5 min), pepper PME in pure form and crude extract was gradually inactivated showing a biphasic inactivation behaviour, indicating the presence of isoenzymes of different thermostability. Pepper samples heated for 15 min showed a maximum of residual PME activity around 55 °C. Isothermal inactivation of pepper PME in purified form and crude extract at pH 5.6 could be described by a biphasic inactivation model for the temperature range studied (62–76 °C). A stable behaviour towards high-pressure/temperature treatments (400–800 MPa/25–60 °C) was observed for crude extract and purified pepper PME. PME in pepper puree samples revealed to be very pressure stable. Mild temperatures combined with pressure treatments seem to increase the extractability from PME in pepper tissue, probably due to the effect on the cell structure.

Keywords

Pectin methylesteraseCapsicum annuumPepper piecesPureeThermal/high-pressure treatments

Copyright information

© Springer-Verlag 2005