Original Paper

European Food Research and Technology

, Volume 222, Issue 3, pp 403-413

First online:

Vitamin D and provitamin D in fish

Determination by HPLC with electrochemical detection
  • Ute OstermeyerAffiliated withFederal Research Centre for Nutrition and Food, Department of Fish Quality Email author 
  • , Thomas SchmidtAffiliated withFederal Research Centre for Nutrition and Food, Department of Fish Quality

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Abstract

A highly-sensitive high-performance liquid chromatographic method for measuring vitamin D3 and provitamin D3 in the edible part of fish was developed. The sample preparation procedure consisted of cold saponification and liquid/liquid extraction of the unsaponifiable matter using a disposable kieselguhr cartridge. For the purification of the sample extracts a semipreparative stage using normal-phase HPLC with UV-detection was applied. A vitamin D and a provitamin D fraction was collected and subsequently quantified by analytical reversed-phase HPLC with electrochemical detection. Internal standardization was used for quantitation. The accuracy of this method was evaluated by recovery experiments. The investigated fish included most of the important fish species consumed in Germany.

Keywords

Vitamin D Provitamin D Fish Kieselguhr cartridges HPLC Electrochemical detection