European Food Research and Technology

, Volume 214, Issue 5, pp 449–454

5'-Nuclease PCR for quantitative event-specific detection of the genetically modified Mon810 MaisGard maize

  • Askild Holck
  • Marc Vaïtilingom
  • Luc Didierjean
  • Knut Rudi
Original paper

DOI: 10.1007/s00217-001-0473-y

Cite this article as:
Holck, A., Vaïtilingom, M., Didierjean, L. et al. Eur Food Res Technol (2002) 214: 449. doi:10.1007/s00217-001-0473-y
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Abstract.

Genetically modified maize is grown extensively in the world today. MaisGard (Monsanto, Yieldgard in the USA) is a genetically modified maize harbouring the Mon810 transformation event. European Community legislation requires that genetically modified organisms (GMOs) be approved before they are placed on the market. Labelling is required when more than 1% of any ingredient of a food originates from a GMO. There is consequently a need for specific, quantitative methods for detection of genetically modified foods. We have determined the DNA sequence of the 5'-flanking region of the Mon810 insert using ligation mediated PCR. A primer probe set overlapping the junction was designed and used in a quantitative, event-specific Taqman 5'-nuclease assay. Mon810 DNA was quantified relative to endogenous maize zein gene DNA. The results were expressed as the percentage of genetically modified Mon810 maize DNA relative to the total content of maize DNA.

Quantitative real time polymerase chain reaction Genetically modified organism detection Event-specific Mon810 MaisGard

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Askild Holck
    • 1
  • Marc Vaïtilingom
    • 2
  • Luc Didierjean
    • 2
  • Knut Rudi
    • 1
  1. 1.MATFORSK, Norwegian Food Research Institute, Osloveien 1, 1430 AAS, Norway
  2. 2.Tepral, Kronenbourg Research Centre, Strasbourg, France