, Volume 356, Issue 1, pp 1-9

Certification of fat-soluble vitamins, carotenoids, and cholesterol in human serum: Standard reference material 968b

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In Standard Reference Material 968b, fat-soluble vitamins and cholesterol in human serum, certified values are provided for cholesterol, retinol, retinyl palmitate, α-tocopherol, trans-β-carotene, total β-carotene (trans plus cis isomers), total α-carotene, and lutein. Non-certified values are also reported for γ-tocopherol (includes β-tocopherol), δ-tocopherol, zeaxanthin, β-cryptoxanthin, trans-lycopene, trans-lycopene, trans-α-carotene, total lycopene, 9-cis-β-carotene, 13-plus 15-cis-β-carotene, and 15-cis-β-carotene. Both certified and non-certified values are based on the agreement among results from three different liquid Chromatographic analytical procedures developed at NIST and from an interlaboratory comparison exercise among institutions that participate in a NIST-managed Micronutrients Measurement Quality Assurance Program. Cholesterol is certified in this material using the NIST isotope dilution/mass spectrometric definitive method.