Fresenius' Journal of Analytical Chemistry

, Volume 356, Issue 1, pp 1–9

Certification of fat-soluble vitamins, carotenoids, and cholesterol in human serum: Standard reference material 968b

  • Jeanice Brown Thomas
  • Margaret C. Kline
  • Susannah B. Schiller
  • Polly M. Ellerbe
  • Lorna T. Sniegoski
  • David L. Duewer
  • Katherine E. Sharpless
Original Paper Reference Materials

DOI: 10.1007/s0021663560001

Cite this article as:
Thomas, J.B., Kline, M.C., Schiller, S.B. et al. Fresenius J Anal Chem (1996) 356: 1. doi:10.1007/s0021663560001

Abstract

In Standard Reference Material 968b, fat-soluble vitamins and cholesterol in human serum, certified values are provided for cholesterol, retinol, retinyl palmitate, α-tocopherol, trans-β-carotene, total β-carotene (trans plus cis isomers), total α-carotene, and lutein. Non-certified values are also reported for γ-tocopherol (includes β-tocopherol), δ-tocopherol, zeaxanthin, β-cryptoxanthin, trans-lycopene, trans-lycopene, trans-α-carotene, total lycopene, 9-cis-β-carotene, 13-plus 15-cis-β-carotene, and 15-cis-β-carotene. Both certified and non-certified values are based on the agreement among results from three different liquid Chromatographic analytical procedures developed at NIST and from an interlaboratory comparison exercise among institutions that participate in a NIST-managed Micronutrients Measurement Quality Assurance Program. Cholesterol is certified in this material using the NIST isotope dilution/mass spectrometric definitive method.

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Jeanice Brown Thomas
    • 1
  • Margaret C. Kline
    • 2
  • Susannah B. Schiller
    • 3
  • Polly M. Ellerbe
    • 1
  • Lorna T. Sniegoski
    • 1
  • David L. Duewer
    • 1
  • Katherine E. Sharpless
    • 1
  1. 1.Analytical Chemistry DivisionNISTGaithersburgUSA
  2. 2.Biotechnology DivisionNISTGaithersburgUSA
  3. 3.Statistical Engineering DivisionNISTGaithersburgUSA