Fresenius' Journal of Analytical Chemistry

, Volume 355, Issue 1, pp 94–95

Spectrophotometric method for the determination of ascorbic acid with iron (III)-1,10-phenanthroline after preconcentration on an organic solvent-soluble membrane filter


  • Xuexin Gu
    • Department of ChemistryCapital Normal University
  • Chunhua Chen
    • Department of ChemistryCapital Normal University
  • Tianze Zhou
    • Department of ChemistryCapital Normal University
Short Communication

DOI: 10.1007/s0021663550094

Cite this article as:
Gu, X., Chen, C. & Zhou, T. Fresenius J Anal Chem (1996) 355: 94. doi:10.1007/s0021663550094


A solvent-soluble membrane filter is proposed for the simple and rapid preconcentration and spectrophotometric determination of ascorbic acid based on the reduction of 1, 10-phenanthroline (phen)-iron (III), which is collected on a nitrocellulose membrane filter as an ion-associate of the cationic complex of tri,phen-iron (II) [ferroin, Fe(phen)32+] with an anionic surfactant (of dodecyl sulfate). The ion-associate collected is dissolved in a small volume of 2-methoxyethanol together with the filter. The colour intensity is measured at 510 nm against the reagent blank and is proportional to the content of ascorbic acid in the range 2.5–50 μg ascorbic acid in 5 ml of solvent with excellent reproducibility (RSD 3.2% for 200 μg 1−1 ascorbic acid), the enrichment factor achieves 100-fold and detection limits better than 2.0 μg 1−1 can be obtained. Diverse components of organic and inorganic compounds normally present in fruits, vegetable, beverages and urine do not interfere. The recoveries of the ascorbic acid added to the samples are quantitative.

Copyright information

© Springer-Verlag 1996