Original paper

Fresenius' Journal of Analytical Chemistry

, Volume 364, Issue 8, pp 727-731

First online:

Optimisation of the analytical detection of EDTA by HPLC in natural waters

  • Stéphanie Loyaux-LawniczakAffiliated withInstitut de Mécanique des Fluides UMR 7507 Université Louis Pasteur-CNRS, 2, rue Boussingault, F-67000 Strasbourg, France e-mail: behra@imf.u-strasbg.fr
  • , Jamaâ DouchAffiliated withInstitut de Mécanique des Fluides UMR 7507 Université Louis Pasteur-CNRS, 2, rue Boussingault, F-67000 Strasbourg, France e-mail: behra@imf.u-strasbg.fr
  • , P. BehraAffiliated withInstitut de Mécanique des Fluides UMR 7507 Université Louis Pasteur-CNRS, 2, rue Boussingault, F-67000 Strasbourg, France e-mail: behra@imf.u-strasbg.fr

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Abstract

Reversed-phase ion-pair liquid chromatography is often used to analyse traces of EDTA in natural water samples. Some perturbations due to the presence of major anions in samples, which can compete for ion pair formation with the Fe-EDTA complex used for the detection, have been observed but never estimated and quantified. A new operational method for determining EDTA in natural water samples is proposed by taking into account anion interactions. Qualitative and quantitative analyses of EDTA were performed with a synthetic water at various ionic strengths. From the effect of the ionic strength controlled by CaCl2, the concentration of the counter-ion tetrabutylammonium bromide (TBA+Br) and the methanol of the mobile phase, optimal analytical conditions were proposed for a better determination of EDTA concentration. The limit of detection without preconcentration and the reproducibility defined as relative standard deviation were 5 μg/L and 3.6% (n = 12), respectively. This method was applied to natural samples.