Fresenius' Journal of Analytical Chemistry

, Volume 369, Issue 3, pp 313–319

Development and analytical applications of multispectral imaging techniques: An overview


  • C. D. Tran
    • Department of Chemistry, Marquette University, P. O. Box 1881, Milwaukee, Wisconsin 53201, USA
Special issue paper

DOI: 10.1007/s002160000653

Cite this article as:
Tran, C. Fresenius J Anal Chem (2001) 369: 313. doi:10.1007/s002160000653


A multispectral imaging spectrometer is an instrument that can simultaneously record spectral and spatial information of a sample. Chemical and physical properties of the sample can be elucidated from such images. By synergistic use of an acousto-optic tunable filter and a progressive scan camera capable of snap shot recording it was possible to develop a novel imaging spectrometer with a spatial resolution of a few microns and which can record, grab and store up to 33 images per second (at a function of time) or 16 images per second (as a function of wavelength). This overview article summarizes the instrumentation development of various imaging spectrometers and their applications including its use as the detector for the determination of identity and sequences of peptides synthesized by the combinatorial solid phase method.

Copyright information

© Springer-Verlag Berlin Heidelberg 2001