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Imaging the activity of nitrate reductase by means of a scanning electrochemical microscope

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Abstract

Scanning electrochemical microscopy (SECM) was used to characterize immobilized nitrate reductase (NaR) from Pseudomonas stutzeri (E.C. 1.7.99.4). Nitrate reductase with membrane fragment was embedded in a polyurethane hydrogel in a capillary and solubilized NaR without membrane fragment was covalently coupled to a diaminoethyl-cellulose-carbamitate film on glass. After systematic studies of possible mediators, SECM feedback imaging of both forms of immobilized NaR was accomplished with methylviologen as redox mediator.

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Authors and Affiliations

  1. Wilhelm-Ostwald-Institute of Physical and Theoretical Chemistry, University of Leipzig, Linnéstrasse 2, D-04103 Leipzig, Germany, , , , , , DE

    J. Zaumseil & G. Wittstock

  2. Institute of Molecular Biotechnology, Beutenbergstrasse 11, D-07745 Jena, Germany, , , , , , DE

    S. Bahrs & P. Steinrücke

Authors
  1. J. Zaumseil
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  2. G. Wittstock
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  3. S. Bahrs
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  4. P. Steinrücke
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Received: 30 November 1999 / Accepted: 12 February 2000

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Zaumseil, J., Wittstock, G., Bahrs, S. et al. Imaging the activity of nitrate reductase by means of a scanning electrochemical microscope. Fresenius J Anal Chem 367, 352–355 (2000). https://doi.org/10.1007/s002160000370

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  • DOI: https://doi.org/10.1007/s002160000370

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