Analytical and Bioanalytical Chemistry

, Volume 409, Issue 12, pp 3067–3076

Label-free visualization of nilotinib-functionalized gold nanoparticles within single mammalian cells by C60- SIMS imaging

  • Anna N. Bloom
  • Hua Tian
  • Christian Schoen
  • Nicholas Winograd
Paper in Forefront

DOI: 10.1007/s00216-017-0262-5

Cite this article as:
Bloom, A.N., Tian, H., Schoen, C. et al. Anal Bioanal Chem (2017) 409: 3067. doi:10.1007/s00216-017-0262-5

Abstract

Obtaining a comprehensive grasp of the behavior and interaction of pharmaceutical compounds within single cells provides some of the fundamental details necessary for more effective drug development. In particular, the changes ensuing in the carrier, drug, and host environment in targeted drug therapy applications must be explored in greater detail, as these are still not well understood. Here, nilotinib-functionalized gold nanoparticles are examined within single mammalian cells with use of imaging cluster secondary ion mass spectrometry in a model study designed to enhance our understanding of what occurs to these particles once that have been internalized. Nilotinib, several types of gold nanoparticles, and the functionalized combination of the two were surveyed and successfully imaged within single cells to determine uptake and performance. Both nilotinib and the gold particle are able to be distinguished and visualized in the functionalized nanoparticle assembly within the cell. These compounds, while both internalized, do not appear to be present in the same pixels of the chemical image, indicating possible cleavage of nilotinib from the particle after cell uptake. The method provided in this work is a direct measurement of uptake and subcellular distribution of an active drug and its carrier within a framework. The results obtained from this study have the potential to be applied to future studies to provide more effective and specific cellular delivery of a relevant pharmaceutical compound.

Keywords

Secondary ion mass spectrometry Targeted drug therapy Nilotinib Gold nanoparticles 

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Anna N. Bloom
    • 1
  • Hua Tian
    • 1
  • Christian Schoen
    • 2
  • Nicholas Winograd
    • 1
  1. 1.Department of ChemistryThe Pennsylvania State UniversityUniversity ParkUSA
  2. 2.Nanopartz Inc.LovelandUSA

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