Analytical and Bioanalytical Chemistry

, Volume 406, Issue 3, pp 757–770

Development and validation of a rapid multiplex ELISA for pyrrolizidine alkaloids and their N-oxides in honey and feed

Authors

  • Michalina Oplatowska
    • Institute for Global Food Security, School of Biological SciencesQueen’s University Belfast
  • Christopher T. Elliott
    • Institute for Global Food Security, School of Biological SciencesQueen’s University Belfast
  • Anne-Catherine Huet
    • Département SantéCentre d’Economie Rurale (CER Groupe)
  • Mark McCarthy
    • Institute for Global Food Security, School of Biological SciencesQueen’s University Belfast
  • Patrick P. J. Mulder
    • RIKILT-Institute of Food SafetyWageningen UR
  • Christoph von Holst
    • European Commission, DG Joint Research CentreInstitute for Reference Materials and Measurements
  • Philippe Delahaut
    • Département SantéCentre d’Economie Rurale (CER Groupe)
  • Hans P. Van Egmond
    • RIKILT-Institute of Food SafetyWageningen UR
    • Institute for Global Food Security, School of Biological SciencesQueen’s University Belfast
Research Paper

DOI: 10.1007/s00216-013-7488-7

Cite this article as:
Oplatowska, M., Elliott, C.T., Huet, A. et al. Anal Bioanal Chem (2014) 406: 757. doi:10.1007/s00216-013-7488-7
Part of the following topical collections:
  1. Rapid Detection in Food and Feed

Abstract

Pyrrolizidine alkaloids (PAs) are a group of plant secondary metabolites with carcinogenic and hepatotoxic properties. When PA-producing plants contaminate crops, toxins can be transferred through the food chain and cause illness in humans and animals, most notably hepatic veno-occlusive disease. Honey has been identified as a direct risk of human exposure. The European Food Safety Authority has recently identified four groups of PAs that are of particular importance for food and feed: senecionine-type, lycopsamine-type, heliotrine-type and monocrotaline-type. Liquid or gas chromatography methods are currently used to detect PAs but there are no rapid screening assays available commercially. Therefore, the aim of this study was to develop a rapid multiplex ELISA test for the representatives of three groups of alkaloids (senecionine, lycopsamine and heliotrine types) that would be used as a risk-management tool for the screening of these toxic compounds in food and feed. The method was validated for honey and feed matrices and was demonstrated to have a detection capability less than 25 μg/kg for jacobine, lycopsamine, heliotrine and senecionine. The zinc reduction step introduced to the extraction procedure allows for the additional detection of the presence of N-oxides of PAs. This first multiplex immunoassay for PA detection with N-oxide reduction can be used for the simultaneous screening of 21 samples for >12 PA analytes. Honey samples (n = 146) from various origins were analysed for PA determination. Six samples were determined to contain measurable PAs >25 μg/kg by ELISA which correlated to >10 μg/kg by LC-MS/MS.

Keywords

MultiplexELISAPyrrolizidine alkaloidsFood and feedHoneyScreening assay

Supplementary material

216_2013_7488_MOESM1_ESM.pdf (95 kb)
ESM 1(PDF 302 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013