Research Paper

Analytical and Bioanalytical Chemistry

, Volume 405, Issue 10, pp 3233-3242

Trace analysis of acetylcholinesterase inhibitors with antipsychotic drugs for Alzheimer’s disease by capillary electrophoresis with on column field-amplified sample injection

  • Yi-Rou WangAffiliated withSchool of Pharmacy, College of Pharmacy, Kaohsiung Medical University
  • , Yuan-Han YangAffiliated withDepartment of Neurology, Kaohsiung Medical University Hospital
  • , Chi-Yu LuAffiliated withDepartment of Biochemistry, College of Medicine, Kaohsiung Medical University
  • , Shun-Jin LinAffiliated withSchool of Pharmacy, College of Pharmacy, Kaohsiung Medical University
  • , Su-Hwei ChenAffiliated withSchool of Pharmacy, College of Pharmacy, Kaohsiung Medical University Email author 

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Abstract

A simple and sensitive capillary zone electrophoresis (CZE) with UV detection (214 nm) was developed and validated for the simultaneous determination of the acetylcholinesterase inhibitors (AChEI), donepezil, and rivastigmine, with antipsychotic drugs in plasma. A sample pretreatment by liquid–liquid extraction and subsequent quantification by CZE with field-amplified sample injection (FASI) was used. The optimum separation for these analytes was achieved in <20 min at 25 °C with a fused-silica capillary column of 60.2 cm × 50 μm I.D. (effective length 50 cm) and a run buffer containing 120 mM phosphate (pH 4.0) with 0.1 % γ-cyclodextrin, 40 % methanol (MeOH), and 0.02 % polyvinyl alcohol as a dynamic coating to reduce analytes’ interaction with the capillary wall. Using phenformin as an internal standard (40.0 ng/mL), the linear ranges of the proposed method for the simultaneous determination of donepezil, rivastigmine, aripiprazole, quetiapine, risperidone, clozapine, ziprasidone, and trazodone were over the range 4.0–80.0 ng/mL, and olanzapine was over the range 1.0–20.0 ng/mL. The method was applied for concentrations monitoring of AChEIs and antipsychotic drugs in ten Alzheimer’s disease patients with behavioral and psychological symptoms of dementia after oral administration of the commercial products.

http://static-content.springer.com/image/art%3A10.1007%2Fs00216-013-6767-7/MediaObjects/216_2013_6767_Figa_HTML.gif
Figure

Electropherogram of tested chemicals in plasma determinations

Keywords

Determination of acetylcholinesterase inhibitors with antipsychotic drugs Liquid–liquid extraction Field-amplified sample injection Alzheimer’s disease