, Volume 405, Issue 10, pp 3117-3123

Cloud point extraction of Δ9-tetrahydrocannabinol from cannabis resin

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Abstract

A cloud point extraction coupled with high performance liquid chromatography (HPLC/UV) method was developed for the determination of Δ9-tetrahydrocannabinol (THC) in micellar phase. The nonionic surfactant “Dowfax 20B102” was used to extract and pre-concentrate THC from cannabis resin, prior to its determination with a HPLC–UV system (diode array detector) with isocratic elution. The parameters and variables affecting the extraction were investigated. Under optimum conditions (1 wt.% Dowfax 20B102, 1 wt.% Na2SO4, T = 318 K, t = 30 min), this method yielded a quite satisfactory recovery rate (~81 %). The limit of detection was 0.04 μg mL−1, and the relative standard deviation was less than 2 %. Compared with conventional solid–liquid extraction, this new method avoids the use of volatile organic solvents, therefore is environmentally safer.