Rapid Communication

Analytical and Bioanalytical Chemistry

, Volume 405, Issue 10, pp 3085-3089

Simultaneous chromatography and electrophoresis: two-dimensional planar separations

  • Peter R. StevensonAffiliated withDepartment of Chemistry, Brigham Young University—Idaho
  • , Bret E. DunlapAffiliated withDepartment of Chemistry, Brigham Young University—Idaho
  • , Paul S. PowellAffiliated withDepartment of Chemistry, Brigham Young University—Idaho
  • , Brae V. PetersenAffiliated withDepartment of Chemistry, Brigham Young University—Idaho
  • , Christopher J. HatchAffiliated withDepartment of Chemistry, Brigham Young University—Idaho
  • , Hung ChanAffiliated withDepartment of Chemistry, Brigham Young University—Idaho
  • , Garret I. StillAffiliated withDepartment of Chemistry, Brigham Young University—Idaho
  • , Michael T. FultonAffiliated withDepartment of Chemistry, Brigham Young University—Idaho
  • , Justin S. McKellAffiliated withDepartment of Chemistry, Brigham Young University—Idaho

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Single-dimension separations are routinely coupled in series to achieve two-dimensional separations, yet little has been done to simultaneously exploit multiple dimensions during separation. In this work, simultaneous chromatography and electrophoresis is introduced and evaluated for its potential to achieve two-dimensional separations. In simultaneous chromatography and electrophoresis, chromatography occurs via capillary action while an orthogonal electric field concurrently promotes electrophoresis in a second dimension. A novel apparatus with a dual solvent reservoir was designed to apply the concurrent electric field. Various compounds were used to characterize the apparatus and technique, i.e., vitamins, amino acids, and dyes. Improved separation is reported with equivalent analysis times in comparison to planar chromatography alone. The feasibility of simultaneously employing chromatography and electrophoresis in two dimensions is discussed.

http://static-content.springer.com/image/art%3A10.1007%2Fs00216-013-6737-0/MediaObjects/216_2013_6737_Figa_HTML.gif
Figure

Separation of eight dyes is improved in comparison to (a) planar chromatography alone when employing (b) simultaneous chromatography and electrophoresis

Keywords

Planar chromatography Amino acids Separations/instrumentation Electrophoresis