Analytical and Bioanalytical Chemistry

, Volume 405, Issue 11, pp 3773–3781

Supramolecular immobilization of glucose oxidase on gold coated with cyclodextrin-modified cysteamine core PAMAM G-4 dendron/Pt nanoparticles for mediatorless biosensor design

  • Paula Díez
  • Ciprian-George Piuleac
  • Paloma Martínez-Ruiz
  • Santiago Romano
  • María Gamella
  • Reynaldo Villalonga
  • José M. Pingarrón
Original Paper

DOI: 10.1007/s00216-012-6491-8

Cite this article as:
Díez, P., Piuleac, CG., Martínez-Ruiz, P. et al. Anal Bioanal Chem (2013) 405: 3773. doi:10.1007/s00216-012-6491-8

Abstract

Cysteamine core polyamidoamine G-4 dendron branched with β-cyclodextrins was chemisorbed on the surface of Au electrodes and further coated with Pt nanoparticles. Adamantane-modified glucose oxidase was subsequently immobilized on the nanostructured electrode surface by supramolecular association. This enzyme electrode was used to construct a reagentless amperometric biosensor for glucose, making use of the electrochemical oxidation of H2O2 generated in the enzyme reaction. The amperometric response of the biosensor was rapid (6 s) and a linear function of glucose concentration between 5 and 705 μmol L−1. The biosensor had a low detection limit of 2.0 μmol L−1, sensitivity of 197 mA mol−1 L cm−2, and retained 94 % of its initial response after storage for nine days at 4 °C.

Keywords

Biosensor β-cyclodextrin Dendrimer Glucose oxidase Platinum nanoparticles Supramolecular complex 

Supplementary material

216_2012_6491_MOESM1_ESM.pdf (161 kb)
ESM 1(PDF 161 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Paula Díez
    • 1
  • Ciprian-George Piuleac
    • 2
  • Paloma Martínez-Ruiz
    • 3
  • Santiago Romano
    • 3
  • María Gamella
    • 1
  • Reynaldo Villalonga
    • 1
  • José M. Pingarrón
    • 1
  1. 1.Department of Analytical Chemistry, Faculty of ChemistryComplutense University of MadridMadridSpain
  2. 2.Department of Chemical Engineering“Gh. Asachi” Technical UniversityIasiRomania
  3. 3.Department of Organic Chemistry I, Faculty of ChemistryComplutense University of MadridMadridSpain

Personalised recommendations