Analytical and Bioanalytical Chemistry

, Volume 403, Issue 6, pp 1641–1649

Elemental imaging of MRI contrast agents: benchmarking of LA-ICP-MS to MRI

  • J. A. T. Pugh
  • A. G. Cox
  • C. W. McLeod
  • J. Bunch
  • M. J. Writer
  • S. L. Hart
  • A. Bienemann
  • E. White
  • J. Bell
Original Paper

DOI: 10.1007/s00216-012-5973-z

Cite this article as:
Pugh, J.A.T., Cox, A.G., McLeod, C.W. et al. Anal Bioanal Chem (2012) 403: 1641. doi:10.1007/s00216-012-5973-z

Abstract

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been used to map the spatial distribution of magnetic resonance imaging (MRI) contrast agents (Gd-based) in histological sections in order to explore synergies with in vivo MRI. Images from respective techniques are presented for two separate studies namely (1) convection enhanced delivery of a Gd nanocomplex (developmental therapeutic) into rat brain and (2) convection enhanced delivery, with co-infusion of Magnevist (commercial Gd contrast agent) and Carboplatin (chemotherapy drug), into pig brain. The LA technique was shown to be a powerful compliment to MRI not only in offering improved sensitivity, spatial resolution and signal quantitation but also in giving added value regarding the fate of administered agents (Gd and Pt agents). Furthermore simultaneous measurement of Fe enabled assignment of an anomalous contrast enhancement region in rat brain to haemorrhage at the infusion site.

Figure

Gd contrast-enhanced MRI image (left) and LA-ICP-MS 157Gd elemental distribution (right) for pig brain hemisphere dosed via convection enhanced delivery.

Keywords

MRI, laser ablation ICP-MS Elemental imaging Fe, Gd, Pt Biological thin sections 

Supplementary material

216_2012_5973_MOESM1_ESM.pdf (148 kb)
ESM 1(PDF 147 kb)

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • J. A. T. Pugh
    • 1
    • 6
  • A. G. Cox
    • 1
  • C. W. McLeod
    • 1
  • J. Bunch
    • 2
  • M. J. Writer
    • 3
  • S. L. Hart
    • 3
  • A. Bienemann
    • 4
  • E. White
    • 4
  • J. Bell
    • 5
  1. 1.Centre for Analytical SciencesUniversity of SheffieldSheffieldUK
  2. 2.School of ChemistryUniversity of BirminghamBirminghamUK
  3. 3.Wolfson Centre for Gene Therapy of Childhood DiseaseUCL Institute of Child HealthLondonUK
  4. 4.School of Clinical Sciences, Southmead HospitalUniversity of BristolBristolUK
  5. 5.Metabolic and Molecular Imaging Group, MRC Clinical Sciences Centre, Imperial College LondonHammersmith HospitalLondonUK
  6. 6.Department of Chemical and Biological EngineeringUniversity of SheffieldSheffieldUK