Analytical and Bioanalytical Chemistry

, Volume 403, Issue 5, pp 1221–1231

Current applications of high-resolution mass spectrometry in drug metabolism studies

Review

DOI: 10.1007/s00216-012-5807-z

Cite this article as:
Meyer, M.R. & Maurer, H.H. Anal Bioanal Chem (2012) 403: 1221. doi:10.1007/s00216-012-5807-z

Abstract

This paper reviews high-resolution mass spectrometry (HRMS) approaches published in 2007–2011 for the elucidation of drug metabolism with a focus on new therapeutics, new drugs of abuse, and doping agents using time-of-flight, single-stage Orbitrap, ion trap Orbitrap, and other Fourier transform MS-based techniques. The present review provides an overview of metabolite-generating systems and assays used, sample preparation techniques, ionization and fragmentation techniques, as well as data mining strategies and software tools which were used in the reviewed papers. Furthermore, HRMS-specific topics such as demand for a certain resolution or a specific mass accuracy are discussed in detail and corresponding recommendations are given. Finally, the advantages and limitations of these methods are discussed.

Keywords

High resolution Mass spectrometry Liquid chromatography Gas chromatography Metabolism 

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and ToxicologySaarland UniversityHomburg (Saar)Germany

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