Analytical and Bioanalytical Chemistry

, Volume 402, Issue 6, pp 2015–2021

Infrared spectroscopic analysis of mononuclear leukocytes in peripheral blood from Alzheimer’s disease patients

Authors

    • Instituto de Estructura de la Materia, CSIC
  • Marina Molina
    • Escuela Universitaria de Optica, Universidad Complutense
  • Miguel Calero
    • Centro Nacional de MicrobiologíaInstituto de Salud Carlos III
    • CIBERNED, Carlos III National Research Institute
  • Félix Bermejo-Pareja
    • Neurology DepartmentHospital 12 de Octubre
    • CIBERNED, Carlos III National Research Institute
  • Pablo Martínez-Martín
    • Alzheimer Disease Research Unit and CIBERNEDFundación CIEN – ISCIII, Centro Alzheimer F. Reina Sofía
  • Isabel Alvarez
    • Instituto Cajal, CSIC
  • Adolfo Toledano
    • Instituto Cajal, CSIC
Paper in Forefront

DOI: 10.1007/s00216-011-5669-9

Cite this article as:
Carmona, P., Molina, M., Calero, M. et al. Anal Bioanal Chem (2012) 402: 2015. doi:10.1007/s00216-011-5669-9

Abstract

Peripheral mononuclear leukocytes from Alzheimer’s disease (AD) patients were analyzed by infrared spectroscopy and their spectroscopic properties were compared with those from age-matched healthy controls. Two-dimensional correlation analysis of mean spectra measured at various disease stages shows that the protein secondary structure from AD patients involves β-sheet enrichment and carbonyl intensity increase relative to healthy controls. The area percentages of β-sheets, which were obtained by using a peak ratio second-derivative spectral treatment, were used for receiver operating characteristic (ROC) analysis to distinguish between patients with AD and age-matched healthy controls. The critical concentration and area under the curve (AUC) were determined by this curve analysis which showed a good performance for this quantitative assay. The results were 90% sensitivity and 90.5% specificity for determinations involving mild and moderate AD patients, and 82.1% sensitivity and 90.5% specificity for determinations involving patients at the three AD stages (mild, moderate, and severe). The AUC was greater than 0.85 in both scenarios. Taken together these results show that healthy controls are distinguished from mild and moderate AD patients better than from patients with severe disease and suggest that this infrared analysis is a promising strategy for AD diagnostics.

Keywords

Infrared spectroscopy Mononuclear leukocytes Alzheimer’s disease Diagnostics

Copyright information

© Springer-Verlag 2012