Analytical and Bioanalytical Chemistry

, Volume 402, Issue 6, pp 1993-2013

First online:

Comprehensive two-dimensional gas chromatography in metabolomics

  • Martin F. AlmstetterAffiliated withInstitute of Functional Genomics, University of Regensburg
  • , Peter J. OefnerAffiliated withInstitute of Functional Genomics, University of Regensburg
  • , Katja DettmerAffiliated withInstitute of Functional Genomics, University of Regensburg Email author 

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One of the major objectives in metabolomics is the identification of subtle changes in metabolite profiles as affected by genetic or environmental factors. Comprehensive two-dimensional gas chromatography (GC × GC) hyphenated to a fast-acquisition mass spectrometer is a well-established analytical technique to study the composition of complex samples due to its enhanced separation capacity, sensitivity, peak resolution, and reproducibility. This review reports applications of GC × GC to metabolomics studies of sample of different types (biofluid, cells, tissue, bacteria, yeast, plants), and discusses its advantages and limitations.


Metabolomics Metabolite profiling Metabolic fingerprinting Comprehensive two-dimensional gas chromatography