Analytical and Bioanalytical Chemistry

, Volume 402, Issue 4, pp 1593–1600

Evaluation of a novel ELISA for serotonin: urinary serotonin as a potential biomarker for depression

  • Mikaela I. Nichkova
  • Han Huisman
  • Paul M. Wynveen
  • David T. Marc
  • Kelly L. Olson
  • Gottfried H. Kellermann
Original Paper

DOI: 10.1007/s00216-011-5583-1

Cite this article as:
Nichkova, M.I., Huisman, H., Wynveen, P.M. et al. Anal Bioanal Chem (2012) 402: 1593. doi:10.1007/s00216-011-5583-1

Abstract

Depression is a common disorder with physical and psychological manifestations often associated with low serotonin. Since noninvasive diagnostic tools for depression are sparse, we evaluated the clinical utility of a novel ELISA for the measurement of serotonin in urine from depressed subjects and from subjects under antidepressant therapy. We developed a competitive ELISA for direct measurement of serotonin in derivatized urine samples. Assay performance was evaluated and applied to clinical samples. The analytical range of the assay was from 6.7 to 425 μg serotonin/g creatinine (Cr). The limit of quantification was 4.7 μg/g Cr. The average recovery for spiked urine samples was 104.4%. Average intra-assay variation was 4.4%, and inter-assay variation was <20%. The serotonin analysis was very specific. No significant interferences were observed for 44 structurally and nonstructurally related urinary substances. Very good correlation was observed between urinary serotonin levels measured by ELISA and liquid chromatography tandem mass spectrometry (LC-MS/MS; ELISA = 1.16 × LC-MS/MS − 53.8; r = 0.965; mean % bias = 11%; n = 18). Serotonin was stable in acidified urine for 30 days at room temperature and at −20 °C. The established reference range for serotonin was 54–366 μg/g Cr (n = 64). Serotonin levels detected in depressed patients (87.53 ± 4.89 μg/g Cr; n = 60) were significantly lower (p < 0.001) than in nondepressed subjects (153.38 ± 7.99 μg/g Cr). Urinary excretion of serotonin in depressed individuals significantly increased after antidepressant treatment by 5-hydroxy-tryptophane and/or selective serotonin re-uptake inhibitor (p < 0.01). The present ELISA provides a convenient and robust method for monitoring urinary serotonin. It is suitable to monitor serotonin imbalances and may be particularly helpful in evaluating antidepressant therapies.

https://static-content.springer.com/image/art%3A10.1007%2Fs00216-011-5583-1/MediaObjects/216_2011_5583_Figa_HTML.gif
Figure

Monitoring urinary serotonin by ELISA in depressed patients under antidepressant therapy by 5-HTP, SSRIs and a combination of both

Keywords

ImmunoassayELISAUrine5-HydroxytryptamineDepressionAntidepressants

Supplementary material

216_2011_5583_MOESM1_ESM.pdf (1.2 mb)
ESM 1(PDF 1.22 MB)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Mikaela I. Nichkova
    • 1
  • Han Huisman
    • 1
  • Paul M. Wynveen
    • 1
  • David T. Marc
    • 2
  • Kelly L. Olson
    • 2
  • Gottfried H. Kellermann
    • 2
  1. 1.Department of Research and DevelopmentPharmasan Labs, Inc.OsceolaUSA
  2. 2.NeuroScience Inc.OsceolaUSA