Analytical and Bioanalytical Chemistry

, Volume 400, Issue 7, pp 2141–2147

Determination of phenylenediamine isomers in hair dyes by coal cinders micro-column extraction and MEKC

  • Yiwei Wu
  • Feng Jiang
  • Lin Chen
  • Jing Zheng
  • Zhenli Deng
  • Qing Tao
  • Jing Zhang
  • Lijuan Han
  • Xiaoshu Wei
  • Aimin Yu
  • Haili Zhang
Original Paper

DOI: 10.1007/s00216-011-4932-4

Cite this article as:
Wu, Y., Jiang, F., Chen, L. et al. Anal Bioanal Chem (2011) 400: 2141. doi:10.1007/s00216-011-4932-4

Abstract

A new micellar electrokinetic chromatography (MEKC) method using beta-cyclodextrins (β-CDs) and 1-butyl-3-methylimidazolium hexafluorophosphates (ionic liquids) as additives was successfully developed for determination of para-, meta-, and ortho-phenylenediamines isomers (p-P, m-P, and o-P) in hair dyes. To improve the sensitivity of the MEKC-UV, a simple and cheap flow injection (FI) technique using a micro-column packed with coal cinders (the by-products from combustion in a boiler) as solid-phase extractant was also investigated. In the presence of 20 mmol L−1 phosphates at pH 5.5, addition of 12 mmol L−1 ionic liquids and 8 mmol L−1 β-CDs greatly improved the separation efficiency. The three analytes could be quantitatively adsorbed by coal cinders, and desorbed readily with 0.15 mL of 0.01 mol L−1 NaOH. Under the optimum conditions, an enrichment factor (EF) of 33.3 was obtained, and determination limits of p-P, m-P, and o-P were 1.97 × 10−7, 0.99 × 10−7, and 0.61 × 10−7 mol L−1, respectively. The adsorption capacities of the coal cinders micro-column for p-P, m-P, and o-P were all 1.20 mg g−1. The presented procedure was successfully applied to the determination of p-P, m-P, and o-P in hair dyes with satisfactory results.

https://static-content.springer.com/image/art%3A10.1007%2Fs00216-011-4932-4/MediaObjects/216_2011_4932_Figa_HTML.gif
Figure

Electropherograms of 0.1 mmol L−1 p-P, m-P, and o-P obtained by a direct MEKC and b SPE-MEKC; SPE conditions: sample solution flow rate 0.30 mL min−1, eluent flow rate 0.45 mL min−1, desorption by 0.15 mL of 0.01 mol L−1 NaOH; MEKC conditions: 20 mmol L−1 phosphates containing 12 mmol L−1 ionic liquid and 8 mmol L−1 β-CD at pH 5.5; injection time 50 s

Keywords

Coal cinders micro-column extractionHair dyeMEKCPhenylenediamine isomers

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Yiwei Wu
    • 1
    • 2
  • Feng Jiang
    • 1
  • Lin Chen
    • 1
  • Jing Zheng
    • 1
  • Zhenli Deng
    • 1
  • Qing Tao
    • 1
  • Jing Zhang
    • 1
  • Lijuan Han
    • 1
  • Xiaoshu Wei
    • 1
  • Aimin Yu
    • 1
  • Haili Zhang
    • 1
  1. 1.Department of Chemistry and Environmental Engineering, Hubei Key Laboratory of Pollutant Analysis and Reuse TechniqueHubei Normal UniversityHuangshiChina
  2. 2.Key Laboratory of Analytical Chemistry for Biology and Medicine (Wuhan University), Ministry of EducationWuhanChina