Analytical and Bioanalytical Chemistry

, Volume 400, Issue 6, pp 1717–1727

Selenoproteins: the key factor in selenium essentiality. State of the art analytical techniques for selenoprotein studies

  • Isabel Lopez Heras
  • Maria Palomo
  • Yolanda Madrid
Review

DOI: 10.1007/s00216-011-4916-4

Cite this article as:
Heras, I.L., Palomo, M. & Madrid, Y. Anal Bioanal Chem (2011) 400: 1717. doi:10.1007/s00216-011-4916-4

Abstract

Selenium is an essential element for human health. The benefits of selenium are many including protection against cancer, heart diseases and other cardiovascular and muscle disorders. Selenium is also helpful in controlling gastrointestinal disorders, enhancing immunity of the human body and reducing age-related diseases. The health-promoting properties of Se are due to vital functions of selenoproteins in which selenium is present as selenocysteine, the 21st amino acid. To date, dozens of selenoprotein families have been described though many have roles that have not been fully elucidated. Selenoproteins research has attracted tremendous interest from different scientific areas. Analytical chemists have not remained indifferent to the attractive features of these unique proteins. Different analytical techniques, such as multidimensional chromatography–inductively coupled plasma mass spectrometry (ICPMS), electrospray (tandem) mass spectrometry (ESI-MS/MS), matrix-assisted laser desorption ionization time-of flight (MALDI-TOF) and sodium dodecyl sulphate polyacrylamide gel electrophoresis–laser ablation inductively coupled plasma mass spectrometry (SDS-PAGE-LA-ICPMS), have been applied to the determination of selenoproteins and selenium-containing proteins. This review describes the best-characterized selenoproteins to date in addition to the major contributions of analytical chemistry to the field of selenoproteins. The article also highlights the challenges of combining elemental and molecular mass spectrometry for the determination of selenoproteins and selenium-containing proteins.

Keywords

Mass spectrometry / ICP-MSGenomics / ProteomicsBioanalytical methodsSpeciation

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Isabel Lopez Heras
    • 1
  • Maria Palomo
    • 1
  • Yolanda Madrid
    • 1
  1. 1.Departamento de Química Analítica. Facultad de Ciencias QuímicasUniversidad Complutense de MadridMadridSpain