Original Paper

Analytical and Bioanalytical Chemistry

, Volume 400, Issue 8, pp 2565-2576

Identification and quantification of flavonoids and ellagic acid derivatives in therapeutically important Drosera species by LC–DAD, LC–NMR, NMR, and LC–MS

  • Martin ZehlAffiliated withDepartment of Pharmacognosy, University of Vienna
  • , Christina BraunbergerAffiliated withInstitute of Chemistry, Hohenheim University
  • , Jürgen ConradAffiliated withInstitute of Chemistry, Hohenheim University
  • , Marija CrnogoracAffiliated withDepartment of Pharmacognosy, University of Vienna
  • , Stanimira KrastevaAffiliated withDepartment of Pharmacognosy, University of Vienna
  • , Bernhard VoglerAffiliated withDepartment of Chemistry, University of Alabama in Huntsville
  • , Uwe BeifussAffiliated withInstitute of Chemistry, Hohenheim University
  • , Liselotte KrennAffiliated withDepartment of Pharmacognosy, University of Vienna Email author 

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Abstract

Droserae herba is a drug commonly used for treatment of convulsive or whooping cough since the seventeenth century. Because of the contribution of flavonoids and ellagic acid derivatives to the therapeutic activity of Droserae herba, an LC–DAD method has been developed for quantification of these analytes in four Drosera species used in medicine (Drosera anglica, D. intermedia, D. madagascariensis, and D. rotundifolia). During elaboration of the method 13 compounds, including three substances not previously described for Drosera species, were detected and unambiguously identified by means of extensive LC–MS and LC–NMR experiments and by off-line heteronuclear 2D NMR after targeted isolation. The most prominent component of D. rotundifolia and D. anglica, 2″-O-galloylhyperoside, with myricetin-3-O-β-glucopyranoside and kaempferol-3-O-(2″-O-galloyl)-β-galactopyranoside, were identified for the very first time in this genus. The LC–DAD method for quantification was thoroughly validated, and enables, for the first time, separation and precise analysis of these analytes in Droserae herba. Simple sample preparation and use of a narrow-bore column guarantee low cost and simplicity of the suggested system, which is excellently suited to quality control of the drug or herbal medicinal products containing this drug.

http://static-content.springer.com/image/art%3A10.1007%2Fs00216-011-4690-3/MediaObjects/216_2011_4690_Figa_HTML.gif
Figure

2″-O-galloylhyperoside - a major compound in Drosera anglica and Drosera rotundifolia

Keywords

Drosera Identification Quantification LC–DAD LC–MS LC–NMR