Analytical and Bioanalytical Chemistry

, Volume 400, Issue 8, pp 2565–2576

Identification and quantification of flavonoids and ellagic acid derivatives in therapeutically important Drosera species by LC–DAD, LC–NMR, NMR, and LC–MS


  • Martin Zehl
    • Department of PharmacognosyUniversity of Vienna
  • Christina Braunberger
    • Institute of ChemistryHohenheim University
  • Jürgen Conrad
    • Institute of ChemistryHohenheim University
  • Marija Crnogorac
    • Department of PharmacognosyUniversity of Vienna
  • Stanimira Krasteva
    • Department of PharmacognosyUniversity of Vienna
  • Bernhard Vogler
    • Department of ChemistryUniversity of Alabama in Huntsville
  • Uwe Beifuss
    • Institute of ChemistryHohenheim University
    • Department of PharmacognosyUniversity of Vienna
Original Paper

DOI: 10.1007/s00216-011-4690-3

Cite this article as:
Zehl, M., Braunberger, C., Conrad, J. et al. Anal Bioanal Chem (2011) 400: 2565. doi:10.1007/s00216-011-4690-3


Droserae herba is a drug commonly used for treatment of convulsive or whooping cough since the seventeenth century. Because of the contribution of flavonoids and ellagic acid derivatives to the therapeutic activity of Droserae herba, an LC–DAD method has been developed for quantification of these analytes in four Drosera species used in medicine (Drosera anglica, D. intermedia, D. madagascariensis, and D. rotundifolia). During elaboration of the method 13 compounds, including three substances not previously described for Drosera species, were detected and unambiguously identified by means of extensive LC–MS and LC–NMR experiments and by off-line heteronuclear 2D NMR after targeted isolation. The most prominent component of D. rotundifolia and D. anglica, 2″-O-galloylhyperoside, with myricetin-3-O-β-glucopyranoside and kaempferol-3-O-(2″-O-galloyl)-β-galactopyranoside, were identified for the very first time in this genus. The LC–DAD method for quantification was thoroughly validated, and enables, for the first time, separation and precise analysis of these analytes in Droserae herba. Simple sample preparation and use of a narrow-bore column guarantee low cost and simplicity of the suggested system, which is excellently suited to quality control of the drug or herbal medicinal products containing this drug.

2″-O-galloylhyperoside - a major compound in Drosera anglica and Drosera rotundifolia



Supplementary material

216_2011_4690_MOESM1_ESM.pdf (395 kb)
ESM 1(PDF 394 kb)

Copyright information

© Springer-Verlag 2011