Analytical and Bioanalytical Chemistry

, Volume 398, Issue 7, pp 2883–2893

Protein analysis of laser capture micro-dissected tissues revealed cell-type specific biological functions in developing barley grains

Authors

  • Stephanie Kaspar
    • Applied Biochemistry GroupLeibniz Institute of Plant Genetics and Crop Plant Research (IPK)
  • Diana Weier
    • Seed DevelopmentLeibniz Institute of Plant Genetics and Crop Plant Research (IPK)
  • Winfriede Weschke
    • Seed DevelopmentLeibniz Institute of Plant Genetics and Crop Plant Research (IPK)
  • Hans-Peter Mock
    • Applied Biochemistry GroupLeibniz Institute of Plant Genetics and Crop Plant Research (IPK)
    • Applied Biochemistry GroupLeibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Original Paper

DOI: 10.1007/s00216-010-4120-y

Cite this article as:
Kaspar, S., Weier, D., Weschke, W. et al. Anal Bioanal Chem (2010) 398: 2883. doi:10.1007/s00216-010-4120-y

Abstract

Both the nucellar projection (NP) and endosperm transfer cells (ETC) of the developing barley grain (harvested 8 days after flowering) were isolated by laser capture micro-dissection combined with pressure catapulting. Protein extracts were analyzed by nanoUPLC separation combined with ESI-Q-TOF mass spectrometry. The majority of the ∼160 proteins identified were involved in translation, protein synthesis, or protein destination. The NP proteome was enriched for stress defense molecules, while proteins involved in assimilate transport and the mobilization of nutrients were common to both the NP and the ETC. The combined qualitative and quantitative protein profiling allowed for the identification of several proteins showing tissue specificity in their expression, which underlines the distinct biological functions of these two tissues within the developing barley grain.

Keywords

Grain development Barley Micro-dissection Nucellar projection Endosperm transfer cells Multiplexed LC-MS

Supplementary material

216_2010_4120_MOESM1_ESM.pdf (511 kb)
ESM 1 (PDF 510 kb)

Copyright information

© Springer-Verlag 2010