Analytical and Bioanalytical Chemistry

, Volume 398, Issue 3, pp 1395–1402

Monitoring of troponin release from cardiomyocytes during exposure to toxic substances using surface plasmon resonance biosensing

  • Henrik Andersson
  • Bertil Kågedal
  • Carl-Fredrik Mandenius
Original Paper

DOI: 10.1007/s00216-010-4041-9

Cite this article as:
Andersson, H., Kågedal, B. & Mandenius, C. Anal Bioanal Chem (2010) 398: 1395. doi:10.1007/s00216-010-4041-9

Abstract

Troponin T (TnT) is a useful biomarker for studying drug-induced toxicity effects on cardiac cells. We describe how a surface plasmon resonance (SPR) biosensor was applied to monitor the release of TnT from active HL-1 cardiomyocytes in vitro when exposed to cardiotoxic substances. Two monoclonal human TnT antibodies were compared in the SPR immunosensor to analyse the TnT release. The detection limit of TnT was determined to be 30 ng/ml in a direct assay set-up and to be 10 ng/ml in a sandwich assay format. Exposure of the cardiomyocytes to doxorubicin, troglitazone, quinidine and cobalt chloride for periods of 6 and 24 h gave significant SPR responses, whereas substances with low toxicity showed insignificant effects (ascorbic acid, methotrexate). The SPR results were verified with a validated immunochemiluminescence method which showed a correlation of r2 = 0.790.

Keywords

Surface plasmon resonanceTroponin TCardiotoxicityIn vitro toxicityHL-1 cardiomyocytesCytotoxicity screening

Abbreviations

cTnT

cardiac troponin T

RU

resonance units

SPR

surface plasmon resonance

TnT

troponin T

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Henrik Andersson
    • 1
  • Bertil Kågedal
    • 2
  • Carl-Fredrik Mandenius
    • 1
  1. 1.Division of Biotechnology/IFMLinköping UniversityLinköpingSweden
  2. 2.Division of Clinical Chemistry, Department of Clinical and Experimental MedicineLinköping UniversityLinköpingSweden