Short Communication

Analytical and Bioanalytical Chemistry

, Volume 398, Issue 3, pp 1249-1255

First online:

A simple method of surface functionalisation for immuno-specific immobilisation of proteins

  • R. P. Kengne-MomoAffiliated withLaboratoire PEC, UMR CNRS 6087, Université du MaineLaboratoire de Chimie Organique, Université de Yaoundé I
  • , Y. L. JeyachandranAffiliated withLaboratoire PEC, UMR CNRS 6087, Université du Maine
  • , A. AssafAffiliated withUniversité de Nantes, UMR CNRS GEPEA 6144, IUT Génie Biologique
  • , C. EsnaultAffiliated withLaboratoire UCO2M, UMR CNRS 6011, Université du Maine
  • , P. DanielAffiliated withLaboratoire PEC, UMR CNRS 6087, Université du Maine Email author 
  • , J. F. PilardAffiliated withLaboratoire UCO2M, UMR CNRS 6011, Université du Maine
  • , M. J. DurandAffiliated withUniversité de Nantes, UMR CNRS GEPEA 6144, IUT Génie Biologique
  • , F. LagardeAffiliated withLaboratoire PEC, UMR CNRS 6087, Université du MaineUniversité de Nantes, IUT Génie Biologique
  • , E. DongoAffiliated withLaboratoire de Chimie Organique, Université de Yaoundé I
    • , G. ThouandAffiliated withUniversité de Nantes, UMR CNRS GEPEA 6144, IUT Génie Biologique

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Abstract

We present a new and advanced methodology, developed for surface functionalisation of gold and to study immobilisation of an immuno-specific system of proteins. A combination of electrochemical quartz crystal microbalance and Raman spectroscopy techniques allowed a complete understanding of the system starting from surface functionalisation and progressing to the functional structure analysis of immobilised proteins. A simple electrochemical procedure was formulated to prepare sulphonyl chloride terminated gold surfaces that form a strong sulphonamide bond with the receptor protein staphylococcal protein A (SpA). On the SpA grafted surfaces, the immobilisation of a human IgG and consecutive binding of an immuno-specific anti-human IgG was observed. The surface functional groups form a strong interaction with SpA without disturbing its functional properties. The native functional structure of SpA and also the IgGs was found to be retained in their immobilised state.

Keywords

Surface functionalisation Protein immobilisation Raman spectroscopy QCM