Analytical and Bioanalytical Chemistry

, Volume 398, Issue 3, pp 1249–1255

A simple method of surface functionalisation for immuno-specific immobilisation of proteins

Authors

  • R. P. Kengne-Momo
    • Laboratoire PEC, UMR CNRS 6087Université du Maine
    • Laboratoire de Chimie OrganiqueUniversité de Yaoundé I
  • Y. L. Jeyachandran
    • Laboratoire PEC, UMR CNRS 6087Université du Maine
  • A. Assaf
    • Université de Nantes, UMR CNRS GEPEA 6144, IUT Génie Biologique
  • C. Esnault
    • Laboratoire UCO2M, UMR CNRS 6011Université du Maine
    • Laboratoire PEC, UMR CNRS 6087Université du Maine
  • J. F. Pilard
    • Laboratoire UCO2M, UMR CNRS 6011Université du Maine
  • M. J. Durand
    • Université de Nantes, UMR CNRS GEPEA 6144, IUT Génie Biologique
  • F. Lagarde
    • Laboratoire PEC, UMR CNRS 6087Université du Maine
    • Université de Nantes, IUT Génie Biologique
  • E. Dongo
    • Laboratoire de Chimie OrganiqueUniversité de Yaoundé I
  • G. Thouand
    • Université de Nantes, UMR CNRS GEPEA 6144, IUT Génie Biologique
Short Communication

DOI: 10.1007/s00216-010-4032-x

Cite this article as:
Kengne-Momo, R.P., Jeyachandran, Y.L., Assaf, A. et al. Anal Bioanal Chem (2010) 398: 1249. doi:10.1007/s00216-010-4032-x

Abstract

We present a new and advanced methodology, developed for surface functionalisation of gold and to study immobilisation of an immuno-specific system of proteins. A combination of electrochemical quartz crystal microbalance and Raman spectroscopy techniques allowed a complete understanding of the system starting from surface functionalisation and progressing to the functional structure analysis of immobilised proteins. A simple electrochemical procedure was formulated to prepare sulphonyl chloride terminated gold surfaces that form a strong sulphonamide bond with the receptor protein staphylococcal protein A (SpA). On the SpA grafted surfaces, the immobilisation of a human IgG and consecutive binding of an immuno-specific anti-human IgG was observed. The surface functional groups form a strong interaction with SpA without disturbing its functional properties. The native functional structure of SpA and also the IgGs was found to be retained in their immobilised state.

Keywords

Surface functionalisation Protein immobilisation Raman spectroscopy QCM

Copyright information

© Springer-Verlag 2010