, Volume 398, Issue 6, pp 2373-2382

Utilization of microparticles in next-generation assays for microflow cytometers

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Abstract

Micron-sized particles have primarily been used in microfabricated flow cytometers for calibration purposes and proof-of-concept experiments. With increasing frequency, microparticles are serving as a platform for assays measured in these small analytical devices. Light scattering has been used to measure the agglomeration of antibody-coated particles in the presence of an antigen. Impedance detection is another technology being integrated into microflow cytometers for microparticle-based assays. Fluorescence is the most popular detection method in flow cytometry, enabling highly sensitive multiplexed assays. Finally, magnetic particles have also been used to measure antigen levels using a magnetophoretic micro-device. We review the progress of microparticle-based assays in microflow cytometry in terms of the advantages and limitations of each approach.

Figure

Multiplexed assays using coded microspheres provide sensitive assays for on-site microflow cytometers

Published in the special issue on Focus on Bioanalysis with Guest Editors Antje J. Baeumner, Günter Gauglitz, Frieder W. Scheller.