Analytical and Bioanalytical Chemistry

, Volume 398, Issue 6, pp 2373–2382

Utilization of microparticles in next-generation assays for microflow cytometers

Review

DOI: 10.1007/s00216-010-3848-8

Cite this article as:
Kim, J.S. & Ligler, F.S. Anal Bioanal Chem (2010) 398: 2373. doi:10.1007/s00216-010-3848-8

Abstract

Micron-sized particles have primarily been used in microfabricated flow cytometers for calibration purposes and proof-of-concept experiments. With increasing frequency, microparticles are serving as a platform for assays measured in these small analytical devices. Light scattering has been used to measure the agglomeration of antibody-coated particles in the presence of an antigen. Impedance detection is another technology being integrated into microflow cytometers for microparticle-based assays. Fluorescence is the most popular detection method in flow cytometry, enabling highly sensitive multiplexed assays. Finally, magnetic particles have also been used to measure antigen levels using a magnetophoretic micro-device. We review the progress of microparticle-based assays in microflow cytometry in terms of the advantages and limitations of each approach.

Figure

Multiplexed assays using coded microspheres provide sensitive assays for on-site microflow cytometers

Keywords

Microparticle Flow cytometry Microfluidics Microflow cytometer Biosensors 

Copyright information

© US Government 2010

Authors and Affiliations

  1. 1.Center for Bio/Molecular Science and EngineeringNaval Research LaboratoryWashingtonUSA