Analytical and Bioanalytical Chemistry

, Volume 397, Issue 6, pp 2109–2116

Synchrotron microangiography studies of angiogenesis in mice with microemulsions and gold nanoparticles

  • Chia-Chi Chien
  • C. H. Wang
  • C. L. Wang
  • E. R. Li
  • K. H. Lee
  • Y. Hwu
  • Chien-Yi Lin
  • Shing-Jyh Chang
  • C. S. Yang
  • Cyril Petibois
  • G. Margaritondo
Original Paper

DOI: 10.1007/s00216-010-3775-8

Cite this article as:
Chien, C., Wang, C.H., Wang, C.L. et al. Anal Bioanal Chem (2010) 397: 2109. doi:10.1007/s00216-010-3775-8

Abstract

We present an effective solution for the problem of contrast enhancement in phase-contrast microangiography, with the specific objective of visualising small (<8 µm) vessels in tumor-related microangiogenesis. Different hydrophilic and hydrophobic contrast agents were explored in this context. We found that an emulsified version of the hydrophobic contrast agents Lipiodol® provides the best contrast and minimal distortion of the circulation and vessel structure. Such emulsions are reasonably biocompatible and, with sizes of 0 ± 0.8 µm, sufficient to diffuse to the smallest vessel and still provide reasonable contrast. We also explored the use of Au nanoparticle colloids that could be used not only to enhance contrast but also for interesting applications in nanomedicine. Both the Lipiodol microemulsions and Au nanoparticle colloids can be conjugated with medicines or cell specific labeling agents and their small size can allow the study of the diffusion of contrast agents through the vessel leakage. This enables direct imaging of drug delivery which is important for cancer treatment.

https://static-content.springer.com/image/art%3A10.1007%2Fs00216-010-3775-8/MediaObjects/216_2010_3775_Figa_HTML.gif
Figure

 

Keywords

MicroradiologyMicroangiographyAngiogenesisMicro-emulsionGold nanoparticlesContrast agent

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Chia-Chi Chien
    • 1
    • 2
  • C. H. Wang
    • 1
  • C. L. Wang
    • 1
  • E. R. Li
    • 1
  • K. H. Lee
    • 1
  • Y. Hwu
    • 1
    • 2
    • 3
  • Chien-Yi Lin
    • 4
  • Shing-Jyh Chang
    • 4
  • C. S. Yang
    • 5
  • Cyril Petibois
    • 6
  • G. Margaritondo
    • 7
  1. 1.Institute of PhysicsAcademia SinicaTaipeiChina
  2. 2.Department of Engineering and System ScienceNational Tsing Hua UniversityHsinchuChina
  3. 3.Institute of Optoelectronic SciencesNational Taiwan Ocean UniversityKeelungChina
  4. 4.Hsinchu Mackay Memorial HospitalHsinchuChina
  5. 5.Center for NanomedicineNational Health Research InstitutesMiaoliChina
  6. 6.Université de Bordeaux 2Talence-CedexFrance
  7. 7.Ecole Polytechnique Fédérale de Lausanne (EPFL)LausanneSwitzerland