Analytical and Bioanalytical Chemistry

, Volume 397, Issue 5, pp 1743–1753

Celiac disease diagnosis and gluten-free food analytical control

Authors

  • Marta Maria Pereira da Silva Neves
    • Faculdade de Farmácia da Universidade do Porto
    • REQUIMTEInstituto Superior de Engenharia do Porto
    • Departamento de Química Física y AnalíticaUniversidad de Oviedo
  • Maria Begoña González-Garcia
    • Departamento de Química Física y AnalíticaUniversidad de Oviedo
  • Hendrikus Petrus Antonius Nouws
    • REQUIMTEInstituto Superior de Engenharia do Porto
  • Cristina Delerue-Matos
    • REQUIMTEInstituto Superior de Engenharia do Porto
  • Alice Santos-Silva
    • Faculdade de Farmácia da Universidade do Porto
    • Instituto de Biologia Molecular e Celular da Universidade do Porto
    • Departamento de Química Física y AnalíticaUniversidad de Oviedo
Review

DOI: 10.1007/s00216-010-3753-1

Cite this article as:
da Silva Neves, M.M.P., González-Garcia, M.B., Nouws, H.P.A. et al. Anal Bioanal Chem (2010) 397: 1743. doi:10.1007/s00216-010-3753-1

Abstract

Celiac disease (CD) is an autoimmune enteropathy, characterized by an inappropriate T-cell-mediated immune response to the ingestion of certain dietary cereal proteins in genetically susceptible individuals. This disorder presents environmental, genetic, and immunological components. CD presents a prevalence of up to 1% in populations of European ancestry, yet a high percentage of cases remain underdiagnosed. The diagnosis and treatment should be made early since untreated disease causes growth retardation and atypical symptoms, like infertility or neurological disorders. The diagnostic criteria for CD, which requires endoscopy with small bowel biopsy, have been changing over the last few decades, especially due to the advent of serological tests with higher sensitivity and specificity. The use of serological markers can be very useful to rule out clinical suspicious cases and also to help monitor the patients, after adherence to a gluten-free diet. Since the current treatment consists of a life-long gluten-free diet, which leads to significant clinical and histological improvement, the standardization of an assay to assess in an unequivocal way gluten in gluten-free foodstuff is of major importance.

Keywords

Celiac diseaseAutoimmuneTransglutaminaseGliadin

Abbreviations

AGA

anti-gliadin antibodies

CD

celiac disease

EIs

electrochemical immunosensores

EMA

endomysial antibodies

ELISA

enzyme-linked immunosorbent assay

Ig

immunoglobulin

ROC

receiver operating characteristics

tTG

tissue transglutaminase

Copyright information

© Springer-Verlag 2010