Analytical and Bioanalytical Chemistry

, Volume 396, Issue 7, pp 2599–2607

Interaction of a commercial lipid dispersion and local anesthetics in human plasma: implications for drug trapping by “lipid-sinks”

Authors

  • Jaana Laine
    • Laboratory of Analytical Chemistry, Department of ChemistryUniversity of Helsinki
  • Jana Lokajová
    • Laboratory of Analytical Chemistry, Department of ChemistryUniversity of Helsinki
    • Department of Physical and Macromolecular Chemistry, Faculty of ScienceCharles University in Prague
  • Jevgeni Parshintsev
    • Laboratory of Analytical Chemistry, Department of ChemistryUniversity of Helsinki
  • Juha M. Holopainen
    • Helsinki Eye Lab, Department of OphthalmologyUniversity of Helsinki
    • Laboratory of Analytical Chemistry, Department of ChemistryUniversity of Helsinki
Original Paper

DOI: 10.1007/s00216-009-3435-z

Cite this article as:
Laine, J., Lokajová, J., Parshintsev, J. et al. Anal Bioanal Chem (2010) 396: 2599. doi:10.1007/s00216-009-3435-z

Abstract

Interactions between Intralipid dispersion and local anesthetics (bupivacaine, prilocaine, and lidocaine) were investigated. The amount of bupivacaine (the most cardiotoxic analyte of the local anesthetics studied) entrapped in Intralipid in the presence of plasma was studied using an off-line filtration and solid phase extraction method combined with capillary zone electrophoresis for quantification of free unbound bupivacaine. To confirm interactions between the analytes and Intralipid at lower concentrations, direct injection mass spectrometry was used. The use of immobilized Intralipid chromatography-atmospheric pressure ionization–ion trap mass spectrometry in the study of interactions between drugs and Intralipid dispersion is demonstrated. Finally, interactions between Intralipid dispersion and local anesthetics were investigated by electrokinetic capillary chromatography. The electrophoretic mobility of the Intralipid dispersed phase was calculated using the iterative procedure and a homologous series of alkyl phenyl benzoates (C1–C6), and the retention factors for the analytes were determined.

Keywords

Electrokinetic capillary chromatographyImmobilized Intralipid chromatographyIntralipidLocal anestheticsMass spectrometry

Copyright information

© Springer-Verlag 2010