Original Paper

Analytical and Bioanalytical Chemistry

, Volume 396, Issue 7, pp 2599-2607

First online:

Interaction of a commercial lipid dispersion and local anesthetics in human plasma: implications for drug trapping by “lipid-sinks”

  • Jaana LaineAffiliated withLaboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki
  • , Jana LokajováAffiliated withLaboratory of Analytical Chemistry, Department of Chemistry, University of HelsinkiDepartment of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague
  • , Jevgeni ParshintsevAffiliated withLaboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki
  • , Juha M. HolopainenAffiliated withHelsinki Eye Lab, Department of Ophthalmology, University of Helsinki
  • , Susanne K. WiedmerAffiliated withLaboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki Email author 

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Abstract

Interactions between Intralipid dispersion and local anesthetics (bupivacaine, prilocaine, and lidocaine) were investigated. The amount of bupivacaine (the most cardiotoxic analyte of the local anesthetics studied) entrapped in Intralipid in the presence of plasma was studied using an off-line filtration and solid phase extraction method combined with capillary zone electrophoresis for quantification of free unbound bupivacaine. To confirm interactions between the analytes and Intralipid at lower concentrations, direct injection mass spectrometry was used. The use of immobilized Intralipid chromatography-atmospheric pressure ionization–ion trap mass spectrometry in the study of interactions between drugs and Intralipid dispersion is demonstrated. Finally, interactions between Intralipid dispersion and local anesthetics were investigated by electrokinetic capillary chromatography. The electrophoretic mobility of the Intralipid dispersed phase was calculated using the iterative procedure and a homologous series of alkyl phenyl benzoates (C1–C6), and the retention factors for the analytes were determined.

Keywords

Electrokinetic capillary chromatography Immobilized Intralipid chromatography Intralipid Local anesthetics Mass spectrometry