Analytical and Bioanalytical Chemistry

, Volume 397, Issue 3, pp 1069–1082

New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches

  • Davy Guillarme
  • Josephine Ruta
  • Serge Rudaz
  • Jean-Luc Veuthey
Review

DOI: 10.1007/s00216-009-3305-8

Cite this article as:
Guillarme, D., Ruta, J., Rudaz, S. et al. Anal Bioanal Chem (2010) 397: 1069. doi:10.1007/s00216-009-3305-8

Abstract

Recent developments in chromatographic supports and instrumentation for liquid chromatography (LC) are enabling rapid and highly efficient separations. Various analytical strategies have been proposed, for example the use of silica-based monolithic supports, elevated mobile phase temperatures, and columns packed with sub-3 μm superficially porous particles (fused core) or with sub-2 μm porous particles for use in ultra-high-pressure LC (UHPLC). The purpose of this review is to describe and compare these approaches in terms of throughput and resolving power, using kinetic data gathered for compounds with molecular weights ranging between 200 and 1300 g mol−1 in isocratic and gradient modes. This study demonstrates that the best analytical strategy should be selected on the basis of the analytical problem (e.g., isocratic vs. gradient, throughput vs. efficiency) and the properties of the analyte. UHPLC and fused-core technologies are quite promising for small-molecular-weight compounds, but increasing the mobile phase temperature is useful for larger molecules, for example peptides.

https://static-content.springer.com/image/art%3A10.1007%2Fs00216-009-3305-8/MediaObjects/216_2009_3305_Figa_HTML.gif
Figure

Recent progress in HPLC technology to increase throughput and resolving power

Keywords

UHPLCUPLCHTLCMonolithFused-coreKinetic plots

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Davy Guillarme
    • 1
  • Josephine Ruta
    • 1
  • Serge Rudaz
    • 1
  • Jean-Luc Veuthey
    • 1
  1. 1.School of Pharmaceutical SciencesUniversity of Geneva, University of LausanneGeneva 4Switzerland