Original Paper

Analytical and Bioanalytical Chemistry

, Volume 396, Issue 2, pp 775-781

First online:

Separation of peptides by HPLC using a surface-confined ionic liquid stationary phase

  • K. R. ChittaAffiliated withDepartment of Chemistry
  • , D. S. Van MeterAffiliated withSpringborn Smithers Laboratories
  • , A. M. StalcupAffiliated withDepartment of Chemistry Email author 

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Abstract

A butylimidazolium bromide surface-confined ionic liquid stationary phase was synthesized in-house. The synthesized phase was investigated for the separation of five peptides (Gly-Tyr, Val-Tyr-Val, leucine enkephalin, methionine enkephalin, and angiotensin-II). The peptides were successfully separated in less than 5 min. The effect of trifluoroacetic acid (TFA) on the separation of peptides was evaluated with results confirming that TFA was not acting as ion-pairing agent in separation of peptides on this phase.

Keywords

Peptides Retention mechanisms Surface-confined ionic liquids HPLC Separations/theory