Analytical and Bioanalytical Chemistry

, Volume 396, Issue 1, pp 151–162

Mass spectrometry imaging of rat brain sections: nanomolar sensitivity with MALDI versus nanometer resolution by TOF–SIMS

Authors

  • Farida Benabdellah
    • Institut de Chimie des Substances NaturellesCNRS UPR 2301
  • Alexandre Seyer
    • Institut de Chimie des Substances NaturellesCNRS UPR 2301
  • Loïc Quinton
    • Laboratoire de spectrométrie de masse, Centre d’Analyse des Résidus en Traces—GIGA-RUniversité de Liège
    • Institut de Chimie des Substances NaturellesCNRS UPR 2301
  • Alain Brunelle
    • Institut de Chimie des Substances NaturellesCNRS UPR 2301
  • Olivier Laprévote
    • Institut de Chimie des Substances NaturellesCNRS UPR 2301
    • IFR 71, Faculté de PharmacieUniversité Paris Descartes
Original Paper

DOI: 10.1007/s00216-009-3031-2

Cite this article as:
Benabdellah, F., Seyer, A., Quinton, L. et al. Anal Bioanal Chem (2010) 396: 151. doi:10.1007/s00216-009-3031-2

Abstract

Mass spectrometry imaging is becoming a more and more widely used method for chemical mapping of organic and inorganic compounds from various surfaces, especially tissue sections. Two main different techniques are now available: matrix-assisted laser desorption/ionizaton, where the sample, preliminary coated by an organic matrix, is analyzed by a UV laser beam; and secondary ion mass spectrometry, for which the target is directly submitted to a focused ion beam. Both techniques revealed excellent performances for lipid mapping of tissue surfaces. This article will discuss similarities, differences, and specificities of ion images generated by these two techniques in terms of sample preparation, sensitivity, ultimate spatial resolution, and structural analysis.

Keywords

Mass spectrometry imagingMALDITOF–SIMSLipidRat brain

Copyright information

© Springer-Verlag 2009