Original Paper

Analytical and Bioanalytical Chemistry

, Volume 396, Issue 1, pp 151-162

Mass spectrometry imaging of rat brain sections: nanomolar sensitivity with MALDI versus nanometer resolution by TOF–SIMS

  • Farida BenabdellahAffiliated withInstitut de Chimie des Substances Naturelles, CNRS UPR 2301
  • , Alexandre SeyerAffiliated withInstitut de Chimie des Substances Naturelles, CNRS UPR 2301
  • , Loïc QuintonAffiliated withLaboratoire de spectrométrie de masse, Centre d’Analyse des Résidus en Traces—GIGA-R, Université de Liège
  • , David TouboulAffiliated withInstitut de Chimie des Substances Naturelles, CNRS UPR 2301 Email author 
  • , Alain BrunelleAffiliated withInstitut de Chimie des Substances Naturelles, CNRS UPR 2301
  • , Olivier LaprévoteAffiliated withInstitut de Chimie des Substances Naturelles, CNRS UPR 2301IFR 71, Faculté de Pharmacie, Université Paris Descartes

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Abstract

Mass spectrometry imaging is becoming a more and more widely used method for chemical mapping of organic and inorganic compounds from various surfaces, especially tissue sections. Two main different techniques are now available: matrix-assisted laser desorption/ionizaton, where the sample, preliminary coated by an organic matrix, is analyzed by a UV laser beam; and secondary ion mass spectrometry, for which the target is directly submitted to a focused ion beam. Both techniques revealed excellent performances for lipid mapping of tissue surfaces. This article will discuss similarities, differences, and specificities of ion images generated by these two techniques in terms of sample preparation, sensitivity, ultimate spatial resolution, and structural analysis.

Keywords

Mass spectrometry imaging MALDI TOF–SIMS Lipid Rat brain