Analytical and Bioanalytical Chemistry

, Volume 394, Issue 1, pp 95–105

Electrochemical quantification of reactive oxygen and nitrogen: challenges and opportunities

Review

DOI: 10.1007/s00216-009-2692-1

Cite this article as:
Borgmann, S. Anal Bioanal Chem (2009) 394: 95. doi:10.1007/s00216-009-2692-1

Abstract

Reactive oxygen species (ROS) and reactive nitrogen species (RNS) play a crucial role in chemical signaling processes of biological cells. Electrochemistry is one of the rare methods able to directly detect these species. ROS and RNS can be monitored in the local microenvironment of cells in real time at the site where the actual signaling takes place. This review presents recent advances made with amperometric electrochemical techniques. Existing challenges for the quantification of ROS and RNS in biological systems are discussed to promote the development of innovative and reliable cell-based assays.

Figure

Reactive oxygen and nitrogen species (ROS & RNS) are produced biological cells. An amperometric sensor is placed in close proximity. The recorded current I is used to determine fluxes of certain species.

Keywords

Cell systems/single cell analysisElectrochemical sensorsBioanalytical methodsReactive oxygen speciesReactive nitrogen speciesCell signaling

Abbreviations

HEPES

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

NOS

nitric oxide synthases

RNS

reactive nitrogen species

ROS

reactive oxygen species

SECM

scanning electrochemical microscope

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Biologisch-Chemische Mikrostrukturtechnik, Fakultät ChemieTechnische Universität DortmundDortmundGermany
  2. 2.ISAS—Institute for Analytical ScienceDortmundGermany