Original Paper

Analytical and Bioanalytical Chemistry

, 393:1943

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Aptamer-based electrochemical biosensor for Botulinum neurotoxin

  • Fang WeiAffiliated withDepartment of Mechanical and Aerospace Engineering, School of Engineering and Applied Science, University of California at Los Angeles
  • , Chih-Ming HoAffiliated withDepartment of Mechanical and Aerospace Engineering, School of Engineering and Applied Science, University of California at Los Angeles Email author 

Abstract

We have developed an aptamer-based electrochemical sensor for detection of Botulinum neurotoxin, where steric hindrance is applied to achieve specific signal amplification via conformational change of the aptamer. The incubation time and potassium concentration of the reaction buffer were found to be key parameters affecting the sensitivity of detection of the recognition of Botulinum neurotoxin by the aptamer. Under optimized experimental conditions, a high signal-to-noise ratio was obtained within 24 h with a limit of detection (LOD) of 40 pg/ml by two standard deviation cutoffs above the noise level.

Keywords

Aptamer Botulinum neurotoxin Biosafety Electrochemical biosensors Steric hindrance Conducting polymer Bioanalytical methods Bioassays Biosensors