Analytical and Bioanalytical Chemistry

, Volume 394, Issue 4, pp 1117–1124

MicroRNA detection by microarray

Review

DOI: 10.1007/s00216-008-2570-2

Cite this article as:
Li, W. & Ruan, K. Anal Bioanal Chem (2009) 394: 1117. doi:10.1007/s00216-008-2570-2

Abstract

MicroRNAs (miRNAs) are a class of small noncoding RNAs ∼22 nt in length that regulate gene expression and play fundamental roles in multiple biological processes, including cell differentiation, proliferation and apoptosis as well as disease processes. The study of miRNA has thus become a rapidly emerging field in life science. The detection of miRNA expression is a very important first step in miRNA exploration. Several methodologies, including cloning, northern blotting, real-time RT-PCR, microRNA arrays and ISH (in situ hybridization), have been developed and applied successfully in miRNA profiling. This review discusses the main existing microRNA detection technologies, while emphasizing microRNA arrays.

Keywords

MicroRNAMicroRNA detectionMicroRNA microarrayMicroRNA labeling

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghaiChina