Analytical and Bioanalytical Chemistry

, 393:1315

Quantitative analysis of DHEA and androsterone in female urine: investigating the effects of menstrual cycle, oral contraception and training on exercise-induced changes in young women

  • M.-L. Bayle
  • C. Enea
  • P. Goetinck
  • F. Lafay
  • N. Boisseau
  • B. Dugué
  • M.-M. Flament-Waton
  • M.-F. Grenier-Loustalot
Original Paper

DOI: 10.1007/s00216-008-2549-z

Cite this article as:
Bayle, ML., Enea, C., Goetinck, P. et al. Anal Bioanal Chem (2009) 393: 1315. doi:10.1007/s00216-008-2549-z
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Abstract

Dehydroepiandrosterone (DHEA) and its metabolite androsterone (A) are natural steroids secreted in high quantities in human body. To assess the influence of oral contraceptives, menstrual cycle phase, and also physical exercise (acute and chronic such as training) on these metabolites excretions, a collection of 28 female urine specimens was organized. A three-extraction-step method was developed, and the analyses were performed by gas chromatography–mass spectrometry using deuterated 19-noretiocholanolone as the internal standard. Sample hydration state was found to be of great importance for kinetic studies, as it directly influenced the concentrations. No influence of menstrual cycle and training was found for androsterone and DHEA. However, oral contraceptive intake lowered DHEA excretion in urine and A seems to be slightly affected by exercise.

Keywords

AndrosteroneDehydroepiandrosteroneDopingFemale physiologyGas chromatography–mass spectrometryPhysical exercise

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • M.-L. Bayle
    • 1
  • C. Enea
    • 2
  • P. Goetinck
    • 1
  • F. Lafay
    • 1
  • N. Boisseau
    • 2
  • B. Dugué
    • 2
  • M.-M. Flament-Waton
    • 1
  • M.-F. Grenier-Loustalot
    • 1
  1. 1.Service Central d’Analyse (USR 59—CNRS)Echangeur de SolaizeSolaizeFrance
  2. 2.Laboratoire des Adaptations Physiologiques aux Activités Physiques (EA3813)Université de PoitiersPoitiersFrance