Capillary electrophoresis–mass spectrometry in urinary proteome analysis: current applications and future developments

  • Jochen Metzger
  • Joost P. Schanstra
  • Harald Mischak
Review

DOI: 10.1007/s00216-008-2309-0

Cite this article as:
Metzger, J., Schanstra, J.P. & Mischak, H. Anal Bioanal Chem (2009) 393: 1431. doi:10.1007/s00216-008-2309-0

Abstract

The development of noninvasive methods for diagnosis and prognosis of many diseases still remains a challenging task in clinical medicine. In recent years, protein separation and detection techniques have been refined such that they allow proteome screening of biological fluids with good reproducibility, accuracy, and real-time performance. The advancements in this field resulted in the initiation and conduction of many clinical studies aimed towards defining sensitive and selective biomarkers for disease or a pathological condition. In this review, we focus on the description of the state of the art proteomic platforms established or adapted to use urine as sample source for biomarker discovery. In the second part of this review, we will give an overview of recent clinical studies that used capillary electrophoresis–mass spectrometry (CE-MS) in urinary proteomics/peptidomics. Owing to its fast and accurate one-step screening method CE-MS allows resolution of the urinary small molecular weight proteome in high-throughput mode. This makes CE-MS well suited for use in large proteomic studies. The fact that urinary proteomic studies are not restricted to renal and urological disorders, but also enable assessment of systemic diseases like cardiovascular or infectious diseases, clearly demonstrates the considerably high potential that urine offers for biomarker discovery and for diagnosis, staging, prognosis, and progression monitoring of diseases.

Keywords

Biomarker discovery Capillary electrophoresis–mass spectrometry Urinary proteome analysis 

Abbreviations

2DE

two-dimensional electrophoresis

BPH

benign prostatic hyperplasia

CAD

coronary artery disease

CE

capillary electrophoresis

CE-MS

CE-coupled mass spectrometry

CKD

chronic kidney disease

DIGE

differential in-gel electrophoresis

DN

diabetic nephropathy

EOF

electroosmotic flow

ESI-TOF-MS

electrospray time-of-flight mass spectrometer

ESRD

end-stage renal disease

FSGS

focal segmental glomerulosclerosis

GVHD

graft versus host disease

HSCT

hematopoietic stem cell transplantation

IgA-N

IgA nephropathy

LC

liquid chromatography

MCD

minimal change disease

MGN

membranous glomerulonephritis

MS

mass spectrometry

MudPIT

multidimensional protein identification technology

Pca

prostate cancer

SDS

sodium dodecylsulfate

SELDI

surface-enhanced laser desorption/ionization

UPJ

ureteropelvic junction obstruction

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jochen Metzger
    • 1
  • Joost P. Schanstra
    • 2
    • 3
  • Harald Mischak
    • 1
  1. 1.Mosaiques diagnostics & therapeuticsHannoverGermany
  2. 2.Department of Renal and Cardiac RemodellingInserm, U858/I2MR, Team #5Toulouse Cedex 4France
  3. 3.Institut de Médecine Moléculaire de RangueilUniversité Toulouse III Paul SabatierToulouseFrance

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