Original Paper

Analytical and Bioanalytical Chemistry

, Volume 391, Issue 5, pp 1793-1800

First online:

Biosensor-based on-site explosives detection using aptamers as recognition elements

  • Eva Ehrentreich-FörsterAffiliated withFraunhofer Institute for Biomedical Technology Email author 
  • , Dagmar OrgelAffiliated withRiNA Network RNA Technologies GmbH
  • , Andrea Krause-GriepAffiliated withFraunhofer Institute for Biomedical Technology
  • , Birgit CechAffiliated withRiNA Network RNA Technologies GmbH
  • , Volker A. ErdmannAffiliated withInstitute for Biochemistry, Free University of Berlin
  • , Frank BierAffiliated withFraunhofer Institute for Biomedical TechnologyInstitute for Biochemistry and Biology, University of Potsdam
  • , F. W. SchellerAffiliated withInstitute for Biochemistry and Biology, University of Potsdam
  • , Martina RimmeleAffiliated withRiNA Network RNA Technologies GmbH

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Reliable observation, detection and characterisation of polluted soil are of major concern in regions with military activities in order to prepare efficient decontamination. Flexible on-site analysis may be facilitated by biosensor devices. With use of fibre-optic evanescent field techniques, it has been shown that immunoaffinity reactions can be used to determine explosives sensitively. Besides antibodies as molecular recognition elements, high-affinity nucleic acids (aptamers) can be employed. Aptamers are synthetically generated and highly efficient binding molecules that can be derived for any ligand, including small organic molecules like drugs, explosives or derivatives thereof. In this paper we describe the development of specific aptamers detecting the explosives molecule TNT. The aptamers are used as a sensitive capture molecule in a fibre-optic biosensor. In addition, through the biosensor measurements the aptamers could be characterised. The advantages of the aptamer biosensor include its robustness, its ability to discriminate between different explosives molecules while being insensitive to other chemical entities in natural soil and its potential to be incorporated into a portable device. Results can be obtained within minutes. The measurement is equally useful for soil that has been contaminated for a long time and for urgent hazardous spills.


Aptamer TNT In-field measurement Biosensor