Analytical and Bioanalytical Chemistry

, Volume 391, Issue 5, pp 1499–1507

Fluorescence polarization immunoassays and related methods for simple, high-throughput screening of small molecules

Review

DOI: 10.1007/s00216-008-1897-z

Cite this article as:
Smith, D.S. & Eremin, S.A. Anal Bioanal Chem (2008) 391: 1499. doi:10.1007/s00216-008-1897-z

Abstract

Fluorescence polarization immunoassay (FPIA) is a homogeneous (without separation) competitive immunoassay method based on the increase in fluorescence polarization (FP) of fluorescent-labeled small antigens when bound by specific antibody. The minimum detectable quantity of FPIAs with fluorescein label (about 0.1 ng analyte) is comparable with chromatography and ELISA methods, although this may be limited by sample matrix interference. Because of its simplicity and speed, FPIA is readily automated and therefore suitable for high-throughput screening (HTS) in a variety of application areas. Systems that involve binding of ligands to receptor proteins are also susceptible to analysis by analogous FP methods employing fluorescent-labeled ligand and HTS applications have been developed, notably for use in candidate drug screening.

Keywords

Fluorescence polarization immunoassayFluorescence polarizationHigh-throughput screeningFood safety monitoringEnvironmental monitoring

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.MicroPharm Ltd, 51-53 Bartholomew CloseSt. Bartholomew’s HospitalLondonUK
  2. 2.Division of Chemical Enzymology, Faculty of ChemistryM.V. Lomonosov Moscow State UniversityMoscowRussia