Analytical and Bioanalytical Chemistry

, Volume 389, Issue 7, pp 2143–2151

Raman spectroscopic study of bacterial endospores

Authors

  • Joke De Gelder
    • Department of Analytical ChemistryGhent University
  • Patsy Scheldeman
    • Technology and Food UnitInstitute for Agricultural and Fisheries Research (ILVO)
  • Karen Leus
    • Department of Analytical ChemistryGhent University
  • Marc Heyndrickx
    • Technology and Food UnitInstitute for Agricultural and Fisheries Research (ILVO)
    • Department of Analytical ChemistryGhent University
  • Luc Moens
    • Department of Analytical ChemistryGhent University
  • Paul De Vos
    • Laboratory of Microbiology (Lm-UGent)Ghent University
Original Paper

DOI: 10.1007/s00216-007-1616-1

Cite this article as:
De Gelder, J., Scheldeman, P., Leus, K. et al. Anal Bioanal Chem (2007) 389: 2143. doi:10.1007/s00216-007-1616-1

Abstract

Endospores and endospore-forming bacteria were studied by Raman spectroscopy. Raman spectra were recorded from Bacillus licheniformis LMG 7634 at different steps during growth and spore formation, and from spore suspensions obtained from diverse Bacillus and Paenibacillus strains cultured in different conditions (growth media, temperature, peroxide treatment). Raman bands of calcium dipicolinate and amino acids such as phenylalanine and tyrosine are more intense in the spectra of sporulating bacteria compared with those of bacteria from earlier phases of growth. Raman spectroscopy can thus be used to detect sporulation of cells by a characteristic band at 1,018 cm–1 from calcium dipicolinate. The increase in amino acids could possibly be explained by the formation of small acid-soluble proteins that saturate the endospore DNA. Large variations in Raman spectra of endospore suspensions of different strains or different culturing conditions were observed. Next to calcium dipicolinate, tyrosine and phenylalanine, band differences at 527 and 638 cm–1 were observed in the spectra of some of the B. sporothermodurans spore suspensions. These bands were assigned to the incorporation of cysteine residues in spore coat proteins. In conclusion, Raman spectroscopy is a fast technique to provide useful information about several spore components.

https://static-content.springer.com/image/art%3A10.1007%2Fs00216-007-1616-1/MediaObjects/216_2007_1616_Figa_HTML.gif
Figure

A difference spectrum between Raman spectra of B. licheniformis LMG 7634 cultured for 6 days and 1 day, together with the reference Raman spectrum of calcium dipicolinate

Keywords

Raman spectroscopyBacteriaEndosporeDipicolinic acidBacillus

Copyright information

© Springer-Verlag 2007