Clinical chemistry without reagents? An infrared spectroscopic technique for determination of clinically relevant constituents of body fluids

  • Gamze Hoşafçı
  • Oliver Klein
  • Gerhard Oremek
  • Werner Mäntele
Original Paper

DOI: 10.1007/s00216-006-0841-3

Cite this article as:
Hoşafçı, G., Klein, O., Oremek, G. et al. Anal Bioanal Chem (2007) 387: 1815. doi:10.1007/s00216-006-0841-3
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Abstract

A spectroscopic method based on attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy has been developed for reagent-free analysis of blood and urine constituents in the clinical laboratory and for point-of-care-applications. Blood plasma, whole blood, and urine were analyzed without any sample preparation, such as drying, concentration, or enrichment. Sample volumes as small as 5 μL (a single drop of blood) can be used. Mathematical models, including partial least-squares regression, were used to construct a prediction model which can calculate the concentration of albumin, cholesterol, glucose, total protein, urea, and triglycerides in whole blood or blood plasma samples and the concentration of urea, uric acid, phosphate and creatinine in urine samples. The absolute precision and reproducibility of the prediction reached is sufficient for routine clinical analysis and is only limited by the precision of the reference analysis used for calibration. This was achieved by use of a large number of calibration samples (approx. 400 for blood samples and approx. 100 for urine samples) carefully selected for physiological and pathological range and for specific disease profiles.

Keywords

Blood analysis Urine analysis Body fluids Attenuated total reflection (ATR) Infrared spectroscopy 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Gamze Hoşafçı
    • 1
  • Oliver Klein
    • 1
  • Gerhard Oremek
    • 2
  • Werner Mäntele
    • 1
  1. 1.Institute of BiophysicsUniversity of FrankfurtFrankfurtGermany
  2. 2.Center of Internal Medicine, Research Department of Laboratory MedicineUniversity Hospital FrankfurtFrankfurtGermany

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