Analytical and Bioanalytical Chemistry

, Volume 387, Issue 5, pp 1643–1648

Study of normal colorectal tissue by FT-Raman spectroscopy

  • P. O. Andrade
  • R. A. Bitar
  • K. Yassoyama
  • H. Martinho
  • A. M. E. Santo
  • P. M. Bruno
  • A. A. Martin
Original Paper

DOI: 10.1007/s00216-006-0819-1

Cite this article as:
Andrade, P.O., Bitar, R.A., Yassoyama, K. et al. Anal Bioanal Chem (2007) 387: 1643. doi:10.1007/s00216-006-0819-1
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Abstract

FT-Raman spectroscopy was employed to study normal human colorectal tissues in vitro with the aim of evaluating the spectral differences of the complex colon mucous in order to establish a characteristic Raman spectrum. The samples were collected from 39 patients, providing 144 spectra for the statistical analysis. The results enable one to estabilish three well-defined spectroscopic groups of non-altered coloretal tissues that were consistently checked by statistical (clustering) and biological (histopathology) analyses: group 1 is represented by samples with the presence of epithelial layer, connective tissue papillae, and smooth muscle tissue; group 2 comprises tissues with epithelial layer and connective tissue papillae; group 3 presented mostly fatty and slack conjunctive tissue. The study reveals the existence of an intrinsic spectral variability for each patient that must be considered when sampling tissues fragments to build a spectral database. This is the first step for future studies and applications of Raman spectroscopy to optical biopsy and diagnosis of colorectal cancer.

Keywords

FT-Raman spectroscopyColorectalCancerOptical biopsy

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • P. O. Andrade
    • 1
  • R. A. Bitar
    • 1
  • K. Yassoyama
    • 1
  • H. Martinho
    • 1
  • A. M. E. Santo
    • 1
  • P. M. Bruno
    • 2
  • A. A. Martin
    • 1
  1. 1.Laboratory of Biomedical Vibrational Spectroscopy, Institute of Research and Development (IP&D)Universidade do Vale do Paraiba (UniVap)São José dos Campos, SPBrazil
  2. 2.Vivalle HospitalSão José dos Campos, SPBrazil