Analytical and Bioanalytical Chemistry

, Volume 386, Issue 3, pp 639–644

Measurement of antibody binding to protein immobilized on gold nanoparticles by localized surface plasmon spectroscopy

  • Kazuhiko Fujiwara
  • Hitoshi Watarai
  • Hideaki Itoh
  • Erika Nakahama
  • Nobuaki Ogawa
Original Paper

DOI: 10.1007/s00216-006-0559-2

Cite this article as:
Fujiwara, K., Watarai, H., Itoh, H. et al. Anal Bioanal Chem (2006) 386: 639. doi:10.1007/s00216-006-0559-2

Abstract

Antibody binding to bovine serum albumin (BSA) and human serum albumin (HSA) immobilized onto gold nanoparticles was studied by means of localized surface plasmon resonance (LSPR) spectroscopy. Amine-modified glass was prepared by self-assembly of amine-terminated silane on substrate, and gold (Au) nanoparticles were deposited on the amine-modified glass substrate. Au nanoparticles deposited on the glass surface were functionalized by BSA and HSA. BSA immobilization was confirmed by LSPR spectroscopy in conjunction with surface-enhanced Raman scattering spectroscopy. Then, LSPR response attributable to the binding of anti-BSA and anti-HSA to BSA- and HSA-functionalized Au nanoparticles, respectively, was examined. Anti-HSA at levels larger than ∼10 nM could be detected by HSA-immobilized chips with LSPR optical response, which was saturated at concentrations greater than ∼650 nM of anti-HSA.

Keywords

Gold nanoparticle Localized surface plasmon resonance Biomolecular sensing Antibody Serum albumin Protein immobilization 

Supplementary material

216_2006_559_MOESM1_ESM.pdf (258 kb)
Supplement 1(PDF 263 kb)

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Kazuhiko Fujiwara
    • 1
  • Hitoshi Watarai
    • 2
  • Hideaki Itoh
    • 1
  • Erika Nakahama
    • 1
  • Nobuaki Ogawa
    • 1
  1. 1.Department of Materials Process & Applied Chemistry for Environments, Faculty of Engineering and Resource ScienceAkita UniversityAkitaJapan
  2. 2.Department of Chemistry, Graduate School of ScienceOsaka UniversityOsakaJapan