Review

Analytical and Bioanalytical Chemistry

, Volume 386, Issue 3, pp 416-426

First online:

Recent developments in microarray-based enzyme assays: from functional annotation to substrate/inhibitor fingerprinting

  • Hongyan SunAffiliated withDepartment of Chemistry, National University of Singapore
  • , Souvik ChattopadhayaAffiliated withDepartment of Biological Sciences, National University of Singapore
  • , Jun WangAffiliated withDepartment of Chemistry, National University of Singapore
  • , Shao Q. YaoAffiliated withDepartment of Chemistry, National University of SingaporeDepartment of Biological Sciences, National University of SingaporeMedicinal Chemistry Program of the Office of Life Sciences, National University of Singapore Email author 

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Abstract

Recent advances in proteomics have provided impetus towards the development of robust technologies for high-throughput studies of enzymes. The term “catalomics” defines an emerging ‘-omics’ field in which high-throughput studies of enzymes are carried out by using advanced chemical proteomics approaches. Of the various available methods, microarrays have emerged as a powerful and versatile platform to accelerate not only the functional annotation but also the substrate and inhibitor specificity (e.g. substrate and inhibitor fingerprinting, respectively) of enzymes. Herein, we review recent developments in the fabrication of various types of microarray technologies (protein-, peptide- and small-molecule-based microarrays) and their applications in high-throughput characterizations of enzymes.

Keywords

Enzyme assay Microarray Catalomics Activity-based probe Activity-based fingerprinting Inhibitor fingerprinting Drug discovery