Towards biochips using microstructured optical fiber sensors

  • Lars Rindorf
  • Poul Erik Høiby
  • Jesper Bo Jensen
  • Lars Hagsholm Pedersen
  • Ole Bang
  • Oliver Geschke
Original Paper

DOI: 10.1007/s00216-006-0480-8

Cite this article as:
Rindorf, L., Høiby, P.E., Jensen, J.B. et al. Anal Bioanal Chem (2006) 385: 1370. doi:10.1007/s00216-006-0480-8

Abstract

In this paper we present the first incorporation of a microstructured optical fiber (MOF) into biochip applications. A 16-mm-long piece of MOF is incorporated into an optic-fluidic coupler chip, which is fabricated in PMMA polymer using a CO2 laser. The developed chip configuration allows the continuous control of liquid flow through the MOF and simultaneous optical characterization. While integrated in the chip, the MOF is functionalized towards the capture of a specific single-stranded DNA string by immobilizing a sensing layer on the microstructured internal surfaces of the fiber. The sensing layer contains the DNA string complementary to the target DNA sequence and thus operates through the highly selective DNA hybridization process. Optical detection of the captured DNA was carried out using the evanescent-wave-sensing principle. Owing to the small size of the chip, the presented technique allows for analysis of sample volumes down to 300 nL and the fabrication of miniaturized portable devices.

Keywords

Microstructured optical fiber Biosensor Miniaturization Lab-on-a-chip Packaging 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Lars Rindorf
    • 1
  • Poul Erik Høiby
    • 2
  • Jesper Bo Jensen
    • 1
  • Lars Hagsholm Pedersen
    • 2
  • Ole Bang
    • 1
  • Oliver Geschke
    • 3
  1. 1.COM·DTU, Department of Communication, Optics and MaterialsTechnical University of DenmarkKongens LyngbyDenmark
  2. 2.Bioneer A/SHørsholmDenmark
  3. 3.MIC·DTU, Department of Micro and NanotechnologyTechnical University of DenmarkKongens LyngbyDenmark

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