Analytical and Bioanalytical Chemistry

, Volume 385, Issue 1, pp 189–196

Study of the bioavailability of selenium in cows’ milk after a supplementation of cow feed with different forms of selenium

  • Óscar Muñiz-Naveiro
  • Raquel Domínguez-González
  • Adela Bermejo-Barrera
  • Pilar Bermejo-Barrera
  • José A. Cocho
  • José M. Fraga
Original Paper

DOI: 10.1007/s00216-006-0364-y

Cite this article as:
Muñiz-Naveiro, Ó., Domínguez-González, R., Bermejo-Barrera, A. et al. Anal Bioanal Chem (2006) 385: 189. doi:10.1007/s00216-006-0364-y

Abstract

The purpose of the work described in this paper was to develop an easy and quick in-vitro method for comparing the bioavailability of selenium in cows’ milk after different cow feed. The study focuses on bioavailability differences resulting from the use of different selenium species (organic selenium as selenised yeast and sodium selenite) for supplementation of forage. A procedure for determination of selenium in cows’ milk and dialysates, by hydride-generation atomic-fluorescence spectrometry (HG-AFS) after microwave-assisted acid digestion, was optimised. The results show it is possible to obtain cows’ milk enriched with selenium at different concentration without altering the original composition of the milk. The bioavailability was statistically greater for cows’ milk obtained after supplementation of forage with organic selenium at levels of 0.4 and 0.5 μg Se g−1 than for that obtained after supplementation with inorganic and organic selenium at levels of 0.2 and 0.3 μg Se g−1.

Keywords

Selenium Cows’ milk In-vitro digestion Selenium bioavailability Hydride-generation atomic-fluorescence spectrometry 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Óscar Muñiz-Naveiro
    • 1
  • Raquel Domínguez-González
    • 1
  • Adela Bermejo-Barrera
    • 1
  • Pilar Bermejo-Barrera
    • 1
  • José A. Cocho
    • 2
  • José M. Fraga
    • 3
  1. 1.Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of ChemistryUniversity of Santiago de CompostelaSantiago de CompostelaSpain
  2. 2.Laboratory of Metabolic and Nutritional DisordersUniversity Clinical HospitalSantiago de CompostelaSpain
  3. 3.Department of PediatricsUniversity Clinical HospitalSantiago de CompostelaSpain